[The production of specific immunoenzyme conjugates to human immunoglobulins by the glutaraldehyde method].

The results of studies aimed at obtaining class-specific conjugates to human immunoglobulins to be used in the enzyme immunoassay (EIA) are presented. At the first stage of the studies purified IgA, IgM and IgG preparations were obtained. These preparations were used for obtaining immunologically active immunosorbents on the basis of bromocyanic Sepharose.

[The development of an immunoenzyme method for determining human secretory IgA].

The results of the work on the development of an enzyme immunoassay (EIA) system for the determination of secretory IgA (S-IgA) are presented. A first, S-IgA was isolated from human colostrum and used as the basis for obtaining biologically active immunosorbent; then antibodies to S-IgA were isolated and the specific conjugate was obtained. The determination of S-IgA was carried out by the method of sandwich EIA.

[The development of methods for obtaining monospecific ingredients for immunoenzyme analysis].

The methods of the modification of Salmonella O- and H-antigens and the preparation of biologically active sorbents on their basis have been developed. The use of these sorbents has permitted the isolation of affinity antibodies with strictly defined specific activity. The work shows the possibility of the successful use of carriers obtained on the basis of porous glass, chemically modified by acrylic copolymers containing activated carboxylic groups, and intended for the immobilization of antigens of both protein and carbohydrate nature.

[Use of an affinity chromatography method for isolating monospecific Salmonella antibodies].

Obtaining antibodies to individual components of Salmonella antigenic complex is highly important for investigations aimed at the study of the antigenic structure of bacteria, their serological identification and the development of diagnostic preparations. The method of obtaining antibodies by the oxidation of Salmonella antigens with sodium periodate and creating immunosorbents based on these antibodies with subsequent affinity chromatography has been developed. Monospecific antibodies thus obtained (O2, O4, O9) have been studied and used as monospecific preparations in the agglutination test, the immunofluorescence test and the immunosorbent assay.

[Isolation of monospecific Salmonella anti-O-4 antibodies by affinity chromatography and their use in immunoenzyme reactions].

An immunosorbent with Salmonella typhimurium 1021 O-antigen has been obtained on the basis of polymer-modified porous glass. Binding has been effected due to the presence of NH2-groups on the carrier and aldehyde groupings on O-antigen previously oxidized with sodium periodate. Specific anti-O-4 antibodies have been isolated with the use of this immunosorbent, and on the basis of these antibodies peroxidase conjugates have been obtained.