Trends in Specific Family Refusals for Pediatric Organ and Tissue Donation: A Cross-Sectional Study.

Background: When a family decides to donate the organs or tissues of their child with brain death, it is necessary to consider which organs or tissues will be donated. This phenomenon presents an ethical dilemma that is underexplored in the scientific literature, making it essential to examine this context to understand how refusals occur within donations.

Objective: To analyze the rates and trends of specific refusals for each organ and tissue from pediatric donors with brain death occurring between 2001 and 2020 in an Organ Procurement Organization in the State of São Paulo, Brazil.

Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry.

In recent years, significant advancements have been achieved in the development of multiplex imaging methodologies for immunophenotyping, enabling a comprehensive characterization of the complexity of tumor microenvironments. Imaging mass cytometry combines the detection of over 40 cellular targets with spatial information, enabling the identification of not only which cells are present in a tissue but also their localization relative to each other. Here, we present an easy-to-implement imaging mass cytometry workflow that ranges from antibody selection and testing to running a full panel.

Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies.

Adoptive cell therapy with tumor-infiltrating lymphocytes (TIL) can mediate tumor regression, including complete and durable responses, in a range of solid cancers, most notably in melanoma. However, its wider application and efficacy has been restricted by the limited accessibility, proliferative capacity and effector function of tumor-specific TIL. Here, we develop a platform for the efficient identification of tumor-specific TCR genes from diagnostic tumor biopsies, including core-needle biopsies frozen in a non-viable format, to enable engineered T cell therapy.

Global research interest and publication trends on guided surgery in implant dentistry: A metrics-based analysis.

Statement Of Problem: Digital surgical guides improve precision by detailing the direction, position, and angle of implants, which reduces surgery time and complications. A bibliometric analysis of guided surgery in implant dentistry is lacking.

Purpose: The aim of this metrics-based analysis was to analyze the trends and key characteristics of articles related to guided surgery in implant dentistry.

Laparoscopy Versus Open Technique for Inguinal Hernia Repair in Children: A Systematic Review and Meta-Analysis.

This study aims to compare operative time, recurrence, and complications between laparoscopic and open techniques for the repair of inguinal hernia in children. Pubmed and Embase databases were systematically searched for studies of pediatric patients who underwent open or laparoscopic inguinal hernia procedures. The main outcomes were operative time, recurrence, and complications.