Multi-omic approach identifies hypoxic tumor-associated myeloid cells that drive immunobiology of high-risk pediatric ependymoma.

Ependymoma (EPN) is a devastating childhood brain tumor. Single-cell analyses have illustrated the cellular heterogeneity of EPN tumors, identifying multiple neoplastic cell states including a mesenchymal-differentiated subpopulation which characterizes the PFA1 subtype. Here, we characterize the EPN immune environment, in the context of both tumor subtypes and tumor cell subpopulations using single-cell sequencing (scRNAseq, n = 27), deconvolution of bulk tumor gene expression (n = 299), spatial proteomics (n = 54), and single-cell cytokine release assays (n = 12).

Biofuels and Sustainability.

Energy security and climate change have cascading effects on the world's burgeoning population in terms of food security, environment, and sustainability. Due to depletion of fossil fuels and undesirable changes of climatic conditions, increase in air and water pollution, mankind started exploring alternate and sustainable means of meeting growing energy needs. One of the options is to use renewable sources of fuel-biofuel.

Assessment of Molecular Diversity in Biofuel Crops.

Sustainable biofuel sources require the new sources of biofuel crops that can be developed into scalable plantation to meet the growing energy demands. Diverse supply sources of bioenergy plantations (edible, nonedible, and perennial grasses) will enable de-risking impact on geography and climate change that humans are likely to face in future. Use of phenotypic descriptors alone does not provide a deep insight into plantation population dynamics and molecular diversity of a biofuel crop.

Simple isolation and characterization of seminal plasma extracellular vesicle and its total RNA in an academic lab.

Extracellular vesicles (EVs) are small membrane-bound sacs, identified in many body fluids of humans. Standard extracellular vesicle separation methods such as differential and ultracentrifugation are very expensive, not affordable in academic labs. So, the current research tried to isolate seminal plasma EVs using polyethylene glycol (PEG) precipitation process.

Generation of an expressed sequence tag (EST) library from salt-stressed roots of Jatropha curcas for identification of abiotic stress-responsive genes.

Improving salinity and drought tolerance of crop plants has been an important aim of modern agricultural development, which depends on understanding the functions of genes expressed during the process of stress adaptation. EST resources are an efficient and cost-effective solution to gene discovery. Jatropha curcas is emerging as the most promising tree oil seed as a source of biodiesel.