Bin2cell reconstructs cells from high resolution Visium HD data.

Summary: Visium HD by 10X Genomics is the first commercially available platform capable of capturing full scale transcriptomic data paired with a reference morphology image from archived FFPE blocks at sub-cellular resolution. However, aggregation of capture regions to single cells poses challenges. Bin2cell reconstructs cells from the highest resolution data (2 μm bins) by leveraging morphology image segmentation and gene expression information.

Monitoring maternal near miss/severe maternal morbidity: A systematic review of global practices.

There is international interest in monitoring severe events in the obstetrical population, commonly referred to as maternal near miss or severe maternal morbidity. These events can have significant consequences for individuals in this population and further study can inform practices to reduce both maternal morbidity and mortality. Numerous surveillance systems exist but we lack a standardized approach.

The Graphics System for the 80's.

Forty years ago, the first General Purpose Raster Graphics Processor made the transition from research project to commercial product. This is the story of the creation of a new graphics system and the startup company that produced it in the early days of raster computer graphics.

Mucinous adenocarcinoma of the umbilicus 8 years following anterior resection for villous adenoma of the rectum.

We present the case of an 80-year-old retired consultant histopathologist who presented to us with a malignant umbilical mass 8years following resection of a sigmoid adenoma. The report details initial investigation and management of the umbilical mass and the subsequently discovered pelvic recurrence. Our conclusions of its origin, as a malignant transformation due to seeding of the original sigmoid adenoma, show the slow progression of some colorectal tumours; and the importance of obtaining a complete specimen intra-operatively.

Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery.

If immunized with an antigen of interest, transgenic mice with large portions of unrearranged human immunoglobulin loci can produce fully human antigen-specific antibodies; several such antibodies are in clinical use. However, technical limitations inherent to conventional transgenic technology and sequence divergence between the human and mouse immunoglobulin constant regions limit the utility of these mice. Here, using repetitive cycles of genome engineering in embryonic stem cells, we have inserted the entire human immunoglobulin variable-gene repertoire (2.