Stimulation of rat autologous mixed lymphocyte reaction with xenogeneic sera and their protein preparations.

Autologous mixed lymphocyte cultures were set up from nylon non-adherent T-enriched lymphocytes and mitomycin C-treated spleen cells of individual ACI/N rats and the effect on the reaction (AMLR) of sera in the culture medium was studied with regard to the xenogeneic nature of the sera. Not only foetal calf serum, but also sera of adult human, horse and swine stimulated the AMLR response, but autologous or rat serum did not. Albumin fractions of these sera were also effective in inducing the AMLR response.

Mixed lymphocyte-autologous tumor cell reaction with primary rat bladder tumors: difficulty in the discrimination of tumor-specific antigen.

The presence of tumor-specific or tumor-associated antigens in primary urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) in ACI/N rats was examined by means of mixed lymphocyte-tumor cell culture. Bladder tumors (papilloma with cancer foci or cancer) induced by BBN treatment for 18 weeks or more (BT), epithelial cells from papillary hyperplasia induced by 6-week BBN treatment (PH), and normal bladder epithelial cells (NBE) were isolated from the bladder and used as stimulator cells after mitomycin C treatment. BT, PH and NBE all strongly stimulated the blastogenesis of the autologous lymphocytes on culture in medium containing fetal calf serum (FCS), but the spleen cells also stimulated the autologous lymphocytes in this medium.

Factors influencing the autologous mixed lymphocyte reaction in rats: effect of xenogeneic serum protein.

Mixed culture of nylon non-adherent (T-enriched) spleen cells or lymph node cells of ACI/N or F344 rats with mitomycin C-treated autologous spleen cells resulted in the increased DNA synthesis of the responder T cells when they were cultured in medium containing foetal calf serum (FCS) or bovine serum albumin. This autologous mixed lymphocyte reaction (AMLR), however, proceeds little when the cells were cultured in medium containing autologous or allogeneic rat serum, irrespective of the responder:stimulator cell ratio and to the culture period. Furthermore, the AMLR response in FCS was greatly reduced if a relatively large number of macrophages were present in the responder cell population.