Heterologous expression of calcium-independent mesophilic α-amylase from Priestia megaterium: Immobilization on genipin-modified multi-walled carbon nanotubes and silica supports to enhance thermostability and catalytic activity.

α-Amylases, constituting a significant share of the enzyme market, are mainly synthesized by the genus Bacillus. Enzymes tailored for specific industrial applications are needed to meet the growing demand across a range of industries, and thus finding new amylases and optimizing the ones that already exist are extremely important. This study reports the successful expression, characterization and immobilization of P.

The Sol-Gel Process, a Green Method Used to Obtain Hybrid Materials Containing Phosphorus and Zirconium.

The sol-gel process is a green method used in the last few decades to synthesize new organic-inorganic phosphorus-containing hybrid materials. The sol-gel synthesis is a green method because it takes place in mild conditions, mostly by using water or alcohol as solvents, at room temperature. Therefore, the sol-gel method is, among others, a promising route for obtaining metal-phosphonate networks.

The Biological Impact of Some Phosphonic and Phosphinic Acid Derivatives on Human Osteosarcoma.

Osteosarcoma malignancy currently represents a major health problem; therefore, the need for new therapy approaches is of great interest. In this regard, the current study aims to evaluate the anti-neoplastic potential of a newly developed phosphinic acid derivative (2-carboxyethylphenylphosphinic acid) and, subsequently, to outline its pharmaco-toxicological profile by employing two different in vitro human cell cultures (keratinocytes-HaCaT-and osteosarcoma SAOS-2 cells), employing different techniques (MTT assay, cell morphology assessment, LDH assay, Hoechst staining and RT-PCR). Additionally, the results obtained are compared with three commercially available phosphorus-containing compounds (P1, P2, P3).

A SEM-EDX Study on the Structure of Phenyl Phosphinic Hybrids Containing Boron and Zirconium.

The SEM-EDX method was used to investigate the structure and morphology of organic-inorganic hybrids containing zirconium, boron and phosphorus compounds, synthesized by the sol-gel method. We started by using, for the first time together, zirconyl chloride hexa-hydrate (ZrOCl·6HO), phenyl phosphinic acid and triethyl borate as precursors and reagents, at different molar ratios. The obtained hybrids showed a very high thermal stability and are not soluble in water or in organic solvents.