USE OF HERBAL IMMUNOTROPIC MEDICINES FOR COMPLEX TREATMENT OF GENERALIZED PERIODONTITIS IN PATIENTS WITH CONCOMITANT CORONARY ARTERY DISEASE.

Objective: The aim: Of this study was to improve the efficiency of complex medicamental treatment of generalized periodontitis (GP) in patients with concomitant CAD using of differentiated immunotropic therapy, especially herbal medicine «Immuno-ton».

Patients And Methods: Material and methods: 130 patients with GP were observed (43 without and 81 with chronic CAD - stable angina, functional classes II-III (CCS)) with detection of oral hygiene indices for Green-Vermillion, inflammation of gums PMA, bleeding of gums PBI, depth of periodontal pockets (determined by direct method). The levels of TNF-α and sPECAM-1 in gingival fluid were detected by ELISA method.

[L-arginine assay with the use of arginase I].

A highly selective and sensitive method for the quantitative determination of L-arginine (Arg) with a fluorescent detection of the reaction product has been developed. The method is based on the use of human liver arginase I isolated from a recombinant producer strain, yeast Hansenula polymorpha, and 2,3-butanedione monoxime, which is used to detect carbamide--the product of enzymatic reactions. The linear concentration range for determining Arg in the final reaction mixture varies from 0.

[Human arginase I from the recombinant yeast Hansenula polymorpha: isolation and characterization of the enzyme].

Purified human arginase I preparations homogeneous in SDS-PAAG test were obtained by the affinity chromatography on the synthesized sorbent L-arginine-macroporous glass. Some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and pH-stability, temperature- and pH-optima of the enzyme. The influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was studied.