Fluorescent probe for the detection of acetylcholinesterase inhibitors using high performance thin layer chromatography effect-directed assay in complex matrices.

In recent years Bioautography has become an efficient bioassay for finding active compounds in complex matrices including extracts of; bacteria, plants or fungi. High Performance Thin Layer Chromatography (HPTLC) is a technique which allows effect-directive analysis (EDA) through the separation and identification of biologically active substances on a Thin Layer Chromatography (TLC) plate and can be run as a high throughput screening assay for enzymes. This paper presents a new bioautography method using a novel fluorescent probe derived from coumarin and its validation with Acetylcholinesterase (AChE) inhibition.

[Thyroplasty by external approach with silastic prosthesis in the treatment of laryngeal paralysis].

From August 1995 to January 1997 8 phonosurgical procedures were performed to correct glottal incompetence. There were 4 men and 4 women, the median age was 42 years. The most common indication for thyroplasty was recurrent or vagal nerve injury (6/8 cases).