Publications by authors named "N Degand"
Multiplex microarray PCR Unyvero BCU system to accelerate relevant antimicrobial treatment in polymicrobial bloodstream infection.
Eur J Clin Microbiol Infect Dis
August 2024
Purpose: To evaluate the performance of a rapid multiplex microarray-based method (Unyvero BCU system, BCU) to identify microorganisms and detect antimicrobial resistance directly from positive blood culture (BC) bottles with polymicrobial growth, and to assess relevance of information provided for timely guidance of polymicrobial bloodstream infection treatment.
Methods: Accuracy, time-to-actionable results and potential impact of BCU on antimicrobial treatment were compared with those of standard of care during a prospective study for the sample analysis (November 2017-November 2018) and a retrospective study for the clinical data analysis and the time-to-result analysis. The study was complemented with an experimental study, based on spiked blood cultures to assess the ability of the method to detect antimicrobial resistance genes.
Article Synopsis
- - The French Society of Microbiology changed the temocillin susceptibility breakpoint from 20 mm to 17 mm, aligning with EU guidelines, prompting an evaluation of its impact on Enterobacterales categorization.
- - A study of 36,416 isolates showed that the new 17 mm breakpoint significantly decreased the resistance rate to temocillin from 11.3% to 4.7%, indicating broader susceptibility among tested bacteria.
- - The findings suggest that the updated breakpoint improves treatment options, particularly for resistant strains like Extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-PE), while measurement biases may arise due to temocillin's stability against resistance.
Objectives: Ceftolozane-tazobactam (C/T) proved its efficacy for the treatment of infections caused by non-carbapenemase producing Pseudomonas aeruginosa and Enterobacterales. Here, we aimed to provide susceptibility data on a large series of Enterobacterales since the revision of EUCAST categorization breakpoints in 2020.
Methods: First, C/T susceptibility was determined on characterized Enterobacterales resistant to third generation cephalosporins (3GCs) (extended spectrum β-lactamase [ESBL] production or different levels of AmpC overexpression) (n = 213) and carbapenem-resistant Enterobacterales (CRE) (n = 259), including 170 carbapenemase producers (CPE).
Background: Current guidelines and literature support the use of therapeutic drug monitoring (TDM) to optimize β-lactam treatment in adult ICU patients.
Objectives: To describe the current practice of β-lactam monitoring in French ICUs.
Methods: A nationwide cross-sectional survey was conducted from February 2021 to July 2021 utilizing an online questionnaire that was sent as an email link to ICU specialists (one questionnaire per ICU).
Article Synopsis
- Lots of bacteria called Enterobacterales are becoming harder to treat because they resist medicine that usually works on them, especially for urinary tract infections (UTIs).
- Scientists studied samples from 2017 to 2018 in France to see how many of these bacteria were resistant to different antibiotics.
- They found that while some medicines had high resistance rates, others, like fosfomycin and nitrofurantoin, still worked well against most strains, even the resistant ones.