Publications by authors named "N Davydova"
Article Synopsis
- The study investigates how different P450 enzymes interact and affect drug metabolism changes due to alcohol by analyzing samples from 23 human liver donors with known alcohol consumption histories.
- Findings show a strong link between alcohol exposure and the metabolism rate of ketamine and amitriptyline, highlighting the increased importance of low-affinity enzymes post-alcohol consumption.
- The research indicates that while CYP3A4 is the main enzyme for metabolizing both drugs, several other enzymes displayed no positive correlation or even negative correlations with ketamine metabolism.
New phase transition of p-cresol studied by DSC analysis and FT-IR spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
We have investigated polymorphism in p-cresol using the FT-IR spectroscopy and differential scanning calorimetry. The present results show that in addition to the well-known two crystalline phases of p-cresol, which melts at 307.6 and 309.
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- DiGeorge syndrome (DGS) is a genetic disorder caused by microdeletions in the 22q11.2 region, leading to a range of developmental abnormalities, with a prevalence of about 1 in 4000 births.
- A new multiplex droplet digital PCR (ddPCR) assay was developed to accurately detect and map these microdeletions by amplifying specific targets and including an internal control.
- The assay proved effective in clinical samples, identifying microdeletions and correlating changes in immune cell counts, indicating its potential for reliable DGS diagnosis using blood samples.
We examined the effect of alcohol consumption and smoking on the abundance of drug-metabolizing enzymes and transporters (DMET) in human liver microsomes (HLM) isolated from liver tissues of 94 donors. Global proteomics analysis was performed and DMET protein levels were analyzed in relation to alcohol consumption levels, smoking history, and sex using non-parametric tests (p-value ≤ 0.05; cutoff of 1.
View Article and Find Full Text PDFIn a search for a reliable, inexpensive, and versatile technique for high-throughput kinetic assays of drug metabolism, we elected to rehire an old-school approach based on the determination of formaldehyde (FA) formed in cytochrome P450-dependent demethylation reactions. After evaluating several fluorometric techniques for FA detection, we chose the method based on the Hantzsch reaction with acetoacetanilide as the most sensitive, robust, and adaptable to high-throughput implementation. Here we provide a detailed protocol for using our new technique for automatized assays of cytochrome P450-dependent drug demethylations and discuss its applicability for high-throughput scanning of drug metabolism pathways in the human liver.
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