Publications by authors named "N D Zurita"
Article Synopsis
- This study aims to quickly and accurately detect the Y132F ERG11p mutation in Candida parapsilosis isolates and to analyze genetic diversity among these isolates for better tracking of fluconazole resistance.!* -
- The research involved 223 isolates from patients across 20 hospitals in Spain and Italy, using newly optimized PCR methods that require no DNA extraction and provide results in as little as 1.5 hours.!* -
- The findings confirm both PCR methods have 100% specificity and sensitivity, with Scheme 1 showing greater genetic diversity compared to Scheme 2, suggesting the use of specific microsatellite markers for better genotyping of resistant strains.!*
Sepsis is a time-dependent disease whose prognosis is influenced by early diagnosis and therapeutic measures. Mortality from sepsis remains high, and for this reason, the guidelines of the Surviving Sepsis Campaign recommend establishing specific care programs aimed at patients with sepsis. We present the results of the application of a hospital model to improve performance in sepsis care, called , with the aim of reducing mortality.
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- The study builds on findings from the CANDIMAD study (2019-2021) regarding antifungal resistance in Madrid, revealing trends of increased fluconazole resistance in 2022 among isolates from blood cultures and intra-abdominal samples.
- A total of 766 isolates were collected, with a notable rise in fluconazole resistance (12.0% overall, 16.0% in blood cultures), largely attributed to specific resistant clones, while resistance to other antifungals remained low.
- The data indicates a concerning spread of fluconazole-resistant genotypes in Madrid, with up to 7 out of 16 hospitals reporting affected cases, highlighting the need for vigilance in antifungal treatment strategies.
Objectives: Antifungal susceptibility testing is mostly conducted on blood-cultured Candida spp isolates. Because the intra-abdominal cavity has been highlighted as a hidden echinocandin-resistant C. glabrata reservoir, we assessed whether testing sequential isolates from a given patient might increase the chances of detecting antifungal resistance.
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