Screening chronic myeloid leukemia neutrophils using a novel 3-Dimensional Spectral Gradient Mapping algorithm on hyperspectral images.

Background and objective Early diagnosis of chronic myeloid leukemia (CML) is important for effective treatment. The high spectral and spatial resolution of hyperspectral cellular or tissue images coupled with image analysis algorithms may provide avenues to detect and diagnose diseases early. Many algorithms have been used to analyze medical hyperspectral image data, each having their own strengths and short-comings.

A comparative evaluation of resin- and varnish-based surface protective agents on glass ionomer cement - a spectrophotometric analysis.

To evaluate and compare the effectiveness of resin- and varnish-based surface protective agents on Glass Ionomer Cement (GIC). The different surface protective agents used were: Vaseline, GC Fuji VARNISH™ (varnish), G-Coat Plus™ (resin) and EQUIA Coat (resin). Thirty-six identical specimens of GIC were made.

Myoblast-derived neuronal cells form glutamatergic neurons in the mouse cerebellum.

Production of neurons from non-neural cells has far-reaching clinical significance. We previously found that myoblasts can be converted to a physiologically active neuronal phenotype by transferring a single recombinant transcription factor, REST-VP16, which directly activates target genes of the transcriptional repressor, REST. However, the neuronal subtype of M-RV cells and whether they can establish synaptic communication in the brain have remained unknown.

Reactivation of death receptor 4 (DR4) expression sensitizes medulloblastoma cell lines to TRAIL.

Object: Apoptosis, a key cellular response to therapeutic agents is often inactivated in tumor cells. In this study, we evaluated the expression of the tumor necrosis family of death receptors, DR4 and DR5, in medulloblastoma tumor samples and cell lines to determine if epigenetic modulation of gene expression could sensitize tumor cell lines to TRAIL-mediated apoptosis.

Methods: Human medulloblastoma samples and cell lines were analyzed for DR4 and DR5 expression by quantitative PCR and immunofluorescence assays.

Neurotrophin receptors and heparanase: a functional axis in human medulloblastoma invasion.

Medulloblastoma (MB) is the most common malignant brain tumor of childhood. Although modern therapy has produced five-year survival rates as high as 70% for some MB patients, this resulted in significant long-term treatment-related morbidity. The cellular mechanisms involved in metastatic spread of medulloblastoma are largely unknown.