Evaluation of ISO enrichment real-time PCR methods with internal amplification control for detection of Listeria monocytogenes and Salmonella enterica in fresh fruit and vegetables.

Aims: To provide with a quick method for qualitative detection, in less than three days, of Salmonella enterica and Listeria monocytogenes in fresh fruit and vegetables.

Methods And Results: The method was based on coupling International Standard Organization (ISO) enrichment to a real-time PCR with internal amplification control (IAC), in a duplex format, without additional DNA purification. The performance was tested on different plant products.

The mundane ailment and the physical self: analysis of the social psychology of health and illness.

Using interview data from adult men and women who participated in an ongoing study of self-care, the research focused on the experience of mundane ailments as a promising topic for research in the social psychology of health and illness. Phenomenological analysis of respondents' accounts of everyday ailments generated concepts of 'the physical self', which expands interactive or dramaturgical concepts, and 'the health biography'. These concepts, when linked to analysis of interaction lead to a more dynamic sociology of health, illness, care and cure.