Publications by authors named "N C J de Wit"
Aging of the brain vasculature plays a key role in the development of neurovascular and neurodegenerative diseases, thereby contributing to cognitive impairment. Among other factors, DNA damage strongly promotes cellular aging, however, the role of genomic instability in brain endothelial cells (EC) and its potential effect on brain homeostasis is still largely unclear. We here investigated how endothelial aging impacts blood-brain barrier (BBB) function by using excision repair cross complementation group 1 (ERCC1)-deficient human brain ECs and an EC-specific Ercc1 knock out (EC-KO) mouse model.
View Article and Find Full Text PDFRNA binding by Periphilin plays an essential role in initiating silencing by the HUSH complex.
Nucleic Acids Res
December 2024
Article Synopsis
- The human silencing hub (HUSH) complex is an epigenetic system that silences retroelements in the genome, primarily through components like TASOR, MPP8, and Periphilin, which work together to facilitate chromatin modification.
- Periphilin is identified as the main RNA-binding component of the HUSH complex, and its N-terminal domain is crucial for both RNA binding and the overall function of HUSH.
- The study demonstrates that Periphilin can exert HUSH-dependent silencing even when artificially tethered to a transcript that normally would not be silenced, highlighting its importance in the complex's mechanism of action.
Article Synopsis
- Scientists want to find out how different diets, like high fiber and fermented foods, can change the bacteria in our guts to help keep us healthy.
- They started a 21-week study with healthy adults to see how these diets affect gut bacteria and to teach people about good eating habits.
- The study had 147 volunteers who followed specific diets, and it showed that it's possible to include community involvement to encourage people to stick with the study and its guidelines.
Clear cell renal cell carcinoma (ccRCC) represents the most common form of kidney cancer and is typified by biallelic inactivation of the von Hippel-Lindau () tumour suppressor gene. Here, we undertake genome-wide CRISPR/Cas9 screening to reveal synthetic lethal interactors of , and uncover that loss of Core Binding Factor β (CBF-β) causes cell death in -null ccRCC cell lines and impairs tumour establishment and growth . This synthetic relationship is independent of the elevated activity of hypoxia inducible factors (HIFs) in -null cells, but does involve the RUNX transcription factors that are known binding partners of CBF-β.
View Article and Find Full Text PDFObjective: The CombiConsultation is an innovative concise clinical pharmacy service by the community pharmacist for patients with a chronic condition. We aimed to identify relevant factors influencing the implementation of the CombiConsultation in Dutch clinical practice.
Methods: A mixed-methods study involving interviews and a questionnaire.