Publications by authors named "N Bright"
Electron tomography can provide additional morphological information not easily obtained by conventional transmission electron microscopy of thin sections. It uses a goniometer stage in the electron microscope to tilt the specimen and collect a series of 2D images from different orientations, which are combined to provide a 3D volume tomogram and a colored reconstruction of the morphological feature(s) of interest. Here we describe the protocols for its use in visualizing changes in organelle morphology after depletion of the SNARE proteins VAMP7 and VAMP8 and to study VAMP7 localization on endolysosomes/lysosomes.
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- α-Synuclein (αSYN) is a key protein linked to diseases like Parkinson's and undergoes a process called phase separation, which is influenced by vesicle-associated membrane protein 2 (VAMP2).
- The phase separation of αSYN is driven by electrostatic interactions between VAMP2's juxtamembrane domain and αSYN's C-terminal region, requiring αSYN to bind to lipid membranes.
- αSYN condensates play a role in synaptic function by sequestering vesicles and attracting proteins complexin-1 and -2, shedding light on their importance in both normal and disease-related synaptic activity.
Article Synopsis
- * The study involved in-depth interviews with 26 bereaved parents in Aotearoa New Zealand to understand their experiences with healthcare services after losing a baby, uncovering themes like impersonal care, hospital restrictions, and limited social support.
- * Findings highlighted that the pandemic intensified feelings of isolation for grieving parents but suggested that empathetic healthcare practices and continuity of care could help alleviate some of these challenges.
Regulation of the luminal pH of late endocytic compartments in continuously fed mammalian cells is poorly understood. Using normal rat kidney fibroblasts, we investigated the reversible assembly/disassembly of the proton pumping V-ATPase when endolysosomes are formed by kissing and fusion of late endosomes with lysosomes and during the subsequent reformation of lysosomes. We took advantage of previous work showing that sucrosomes formed by the uptake of sucrose are swollen endolysosomes from which lysosomes are reformed after uptake of invertase.
View Article and Find Full Text PDFImportance: People with serious mental illness (SMI), defined as a diagnosis of schizophrenia spectrum disorder, bipolar disorder, or disabling major depressive disorder) die approximately 10 to 25 years earlier than the general population.
Objective: To develop the first-ever lived experience-led research agenda to address early mortality in people with SMI.
Evidence Review: A virtual 2-day roundtable comprising 40 individuals convened on May 24 and May 26, 2022, and used a virtual Delphi method to arrive at expert group consensus.