Publications by authors named "N Borth"
We present the first use of a bioengineered mammalian transposase system derived from Myotis lucifugus (bMLT) for integration of expression vectors into the CHO genome, focusing on GFP and trastuzumab production. Initially, CHO-K1 cells are transfected with a GFP reporter and varying amounts of bMLT DNA or mRNA. GFP expression is monitored over 17 weeks without selective pressure.
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- Biomarkers play a critical role in predicting disease susceptibility and treatment responses, and they are increasingly valuable in the biomanufacturing of therapeutic proteins by enabling data-driven approaches during cell line and process development.
- Despite the reliance on large screening methods for developing productive cell lines, there is a pressing need to transition to more rational, data-informed strategies as production limits are reached.
- The review discusses various biological molecules as biomarkers, their detection techniques, and challenges faced, while also exploring their applications and limitations in cell line and bioprocess development.
Improving current cell line development workflows can either focus on increasing the specific productivity of the cell lines or shortening timelines to reach the clinic as fast as possible. In this work, using the Beacon platform, we have combined two distinct protocols - early cloning with low-viability pools, and IgG membrane staining-, to concomitantly reach both objectives, and generate highly productive CHO clones in shorter timelines. Fast-sorting approaches using low-viability pools in combination with the Beacon platform have recently been reported to shorten CLD timelines.
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- Mammalian cells use specific mechanisms to control gene expression, which ultimately affects their characteristics or phenotype.
- Modulating these cellular phenotypes is important for understanding diseases and improving the production of biopharmaceuticals, with CHO and HEK293 cells being key players in therapeutic protein production.
- Recent advancements emphasize the need for precise regulation of gene expression, rather than uncontrolled alterations, and highlight synthetic biology tools that allow for fine-tuned and inducible gene control across various regulatory layers.