Case: We present the case of a 33-year-old World Cup giant slalom skier treated by endoscopically assisted proximal vastus lateralis (VL) release for persistent lateral thigh pain, due to a VL proximal myo-aponeurosis rupture. At the completion of rehabilitation, he was able to return to Olympic-level competition following surgery and was still symptom free at the 3-year follow-up.
Conclusion: The unusual location of this injury and the symptoms and physical examination are of interest.
We investigated the efficiency of target-enriched long-read sequencing (TELSeq) for detecting antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) within complex matrices. We aimed to overcome limitations associated with traditional antimicrobial resistance (AMR) detection methods, including short-read shotgun metagenomics, which can lack sensitivity, specificity, and the ability to provide detailed genomic context. By combining biotinylated probe-based enrichment with long-read sequencing, we facilitated the amplification and sequencing of ARGs, eliminating the need for bioinformatic reconstruction.
View Article and Find Full Text PDFUnderstanding the genetic basis of antimicrobial resistance is crucial for developing effective mitigation strategies. One necessary step is to identify the antimicrobial resistance genes (ARGs) within a microbial population, referred to as the resistome, as well as the mobile genetic elements (MGEs) harboring ARGs. Although shotgun metagenomics has been successful in detecting ARGs and MGEs within a microbiome, it is limited by low sensitivity.
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