Polyamines and enzymes of polyamines metabolism in the cartilage during embryonic development.

1. In chicken embryo cartilage putrescine levels, maximal at day 8, fall by day 16 to a four-fold lower value, which remains unchanged through hatching and in the 12-day-old chick. 2.

On the effect of aliphatic saturated dicarboxylic acids on anaerobic glycolysis in chicken embryo.

Oxalic, succinic, glutaric and pimelic acid (5 mM) had no effect on lactate formation from glucose if added to a crude extract of chicken embryo at the same time as substrate and cofactors; conversely malonic, adipic, suberic, azelaic and sebacic acid had an inhibitory effect ranging from 20% to 35%. When the enzyme preparation was pre-incubated with the dicarboxylic acids for one hour before beginning the experiments, all compounds tested, with the exception of succinate, inhibited anaerobic glycolysis. Hexokinase activity was significantly reduced by saturated dicarboxylic acids from C3 to C10, but not by oxalic acid.

Rat lung hexokinase: some aspects of the metabolic and hormonal regulation of its subcellular distribution.

Addition of glucose-6-phosphate (0.03-0.06 mM) to a suspension of rat lung mitochondria promotes solubilization of 30 to 85% of the bound form of hexokinase.