Publications by authors named "N B Gorkina"

Characterization of the microbiomes of pre-launch spacecraft in spacecraft assembly facilities is an important step in keeping crews healthy during journeys that can last several hundred days in small artificial environments in space. , a foodborne pathogenic bacterium, has the potential to be a significant source of food contamination in such environments. This bacterium is a spore-forming bacteria that resists different antimicrobial treatments in cleanrooms where spacecraft are assembled.

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E. coli cells were immobilized in polyacrylamide cryogel by three ways: (1) introduction of cells in the reaction mixture followed by cryopolymerization; (2) the filling of the cryogel pores followed by cell fixation with diluted glutaric dialdehyde (GDA), and (3) the filling of the macropores of the polymeric matrix with modified surface. The ultrastructure of the gels and immobilized cells as well as distribution of attachment of the cells immobilized by different techniques were studied.

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The ultrastructural organization of the cell wall surface was studied in the fungus Aspergillus terreus 17P growing on different substrates: glucose, a sodium salt of carboxymethyl cellulose (CMC) which is a soluble derivative of cellulose, ground straw. Cells grown on soluble substrates (glucose and Na-CMC) had a smooth surface in the logarithmic growth phase. Cells grown on straw, a solid insoluble substrate, released a substance of a polysaccharide nature which formed a thick fibrillar network on the surface.

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