ALTERATIONS OF RBC MEMBRANE PROTEINS IN DIABETIC PATIENTS WITH AND WITHOUT PERIODONTITIS.

The RBC membrane is considered as a key element in their rheology. The rheological properties of RBCs significantly depend on their membranes properties - deformability. The essential contribution of integral membrane proteins in establishing/maintaining membrane stability is due to their ability to anchor the membrane skeleton to the lipid bilayer, their capacity to bind and stabilize membrane lipids, and their ability to influence and regulate local membrane curvature.

β2-adrenergic regulation of T lymphocites function (in vitro study).

The aim of our study was to establish the influence of β2AR agonists and antagonists on Th1/Th2 subpopulation balance in intact and activated CD4+ T lymphocyte. Jurkat leukemic T cell line was used as a model for studying T cell activation conditions under the influence of β2AR ligands. As follows from the results of our studies, after the influence of β2AR agonist isoproterenol on intact Jurkat cells expression of IL-2 was not changed in comparison to control level.

Inflammatory markers of gallstones disease in menopausal women.

Among the factors contributing to the gallstones formation an important role belongs to the inflammation. The goal of our research - determination alterations of the redox balance and cytokines (IL-1α, IL-6, IL-8, TNF-α) content in the blood postmenopausal women with gallstone disease. 58 menopausal women with gallstone disease, who had been admitted to the LTD "1-st Clinic" (Tbilisi, Georgia) during 2009-2011 were studied.

[The role of neuroendocrine mediators in regulatory activity of T-cells].

The aim of the study was to establish the role of some neuroendocrine mediators (agonists and antagonists of β-adrenergic receptors, progesterone) in regulating T-cells activity. Studies conducted on the culture of leukemiatransformed T-cells (Jurkat cells) (DSMZ-Deutshe Sammulung von Mikroorganismen und Zellkulturen (Germany)). Jurkat Cells (4 x 105 cells/ml) stimulated with 50 µg/ml fitogemaglutinin A (PHA) at 370С for 5 minutes and then incubated for 24 hours alone, or together with β- adrenergic receptors agonist izopretonolom (at dose of 10-5 M, 10-6 M), an antagonist, propranolol (dose of 10-5 M, 10-6 M) and progesterone (dose 0,07 µl, 0,7 µl) added to the incubation medium.