Impact of coma duration on functional outcomes at discharge and long-term survival after cardiac arrest.

Introduction: Awakening from coma is crucial for survivors of cardiac arrest, though coma duration is variable. We tested the association of coma duration with short-term functional recovery and long-term survival after cardiac arrest.

Methods: In this retrospective cohort study, we identified post-arrest patients who were comatose on presentation but awakened during hospitalization.

Clinical use of autologous cell-based therapies in an evolving regulatory landscape: A survey of patient experiences and perceptions.

: Clinical treatments involving autologous cell-based therapies (ACBT) remain prevalent despite a lack of scientific backing and an evolving regulatory landscape aimed at assessing their safety and efficacy for clinical adoption. This study seeks to assess patients' experiences and perceptions of clinical treatments involving ACBT and their knowledge and views of the regulatory context and associated governance issues. : An anonymous online survey of 181 participants who have been treated or are in the process of being treated with ACBT was conducted.

The putative prenyltransferase Nus1 is required for filamentation in the human fungal pathogen Candida albicans.

Candida albicans is a major fungal pathogen of humans that can cause serious systemic infections in vulnerable immunocompromised populations. One of its virulence attributes is its capacity to transition between yeast and filamentous morphologies, but our understanding of this process remains incomplete. Here, we analyzed data from a functional genomic screen performed with the C.

Fluorescence Microscopy with Deep UV, Near UV, and Visible Excitation for Detection of Microorganisms.

We report a simple, inexpensive design of a fluorescence microscope with light-emitting diode (LED) excitation for detection of labeled and unlabeled microorganisms in mineral substrates. The use of deep UV (DUV) excitation with visible emission requires no specialized optics or slides and can be implemented easily and inexpensively using an oblique illumination geometry. DUV excitation (<280 nm) is preferable to near UV (365 nm) for avoidance of mineral autofluorescence.