Quantification of antioxidants by using chlorpromazine hydrochloride: application of the method to food and medicinal plant samples.

Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid-ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λ(max) of 530 nm.

Quantification and evaluation of kinetic bio-catalytic pathway of horseradish peroxidase in an electron mediated reaction system and its applications in plant extracts.

The intermolecular coupling of 2,5-dimethoxyaniline (DMA) as mediated electron transfer reaction in presence of H(2)O(2) and peroxidase in acetate buffer of pH 4.2 resulting green colored product having maximum absorption at λ(max)=740 nm was investigated by spectrophotometer. Under optimum conditions, linearity range for the quantification of H(2)O(2) was 2.

Amaranth dye in the evaluation of bleaching of cerium (IV) by antioxidants: application in food and medicinal plants.

A simple, low-cost, sensitive, and diversely applicable spectrophotometric method for the determination of total antioxidant capacity of several medicinal plants and food has been developed. The method is based on the bleaching of cerium (IV) by antioxidants and dye in slightly acid medium at room temperature. The unbleached dye, imparting pink color to the solution, is measured at λ(max) 530 nm which is directly proportional to the antioxidant concentration.

Spectrophotometric method for the determination of chromium (VI) in water samples.

A simple and sensitive spectrophotometric method for the determination of chromium has been developed. The method is based on the diazotization of Dapsone in hydroxylamine hydrochloride medium and coupling with N-(1-Napthyl) Ethylene Diamine Dihydrochloride by electrophilic substitution to produce an intense pink azo-dye, which has absorption maximum at 540 nm. The Beer's law is obeyed from 0.