Publications by authors named "N Amerio"

Introduction: Fungal mitogenomes exhibit remarkable variation in conformation, size, gene content, arrangement and expression, including their intergenic spacers and introns.

Methods: The complete mitochondrial genome sequence of the mycoparasitic fungus was determined using the Illumina next-generation sequencing technology. We used data from our recent Illumina NGS-based project of genome sequencing to study its mitochondrial genome.

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Animal and technical models often require repeated anaesthetic administrations for surgical procedures. As there is evidence for immunomodulatory effects of anaesthesia, the effects of repeated exposure to sevoflurane anaesthesia on the immune response in mice were studied. Sevoflurane was administered in vivo under conditions that simulate those in clinical procedures.

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Based on the immunomodulatory effects of anesthesia and surgery, a study was undertaken to assess the effect of sevoflurane anesthesia on the immune system in a murine model without surgery. Adult male mice were anesthetized with 3% sevoflurane (1.2 minimal alveolar concentration, MAC) in oxygen for 40 min, whereas nontreated animals served as controls.

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Female CBi mice subjected to multiple exposures to halothane inhalation anesthesia before mating were investigated for the potential effects of such intervention on a specific antibody response mounted by them and their offspring. An assessment of the toxicologic and reproductive performance of female mice undergoing anesthesia was also performed. Adult female mice received three episodes of halothane anesthesia at weekly intervals.

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Article Synopsis
  • The study examines how repeated exposure to the anesthetic halothane affects the immune system in mice, both in living organisms (in vivo) and in isolated cells (in vitro).
  • Results showed that halothane increased specific antibody-secreting B-cells without changing the overall immune response to an antigen, while also reducing the number of certain immune cell types in the spleen.
  • The findings suggest that halothane can alter immune responses and cell composition, highlighting the need for careful selection of anesthetics in physiological research to avoid biasing results.
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