Publications by authors named "N Adamczyk"
Background: Osteoarthritis (OA) is a painful degenerative joint disease and a leading source of years lived with disability globally due to inadequate treatment options. Neuroimmune interactions reportedly contribute to OA pain pathogenesis. Notably, in rodents, macrophages in the DRG are associated with onset of persistent OA pain.
View Article and Find Full Text PDFA major barrier that hampers our understanding of the precise anatomic distribution of pain sensing nerves in and around the joint is the limited view obtained from traditional two dimensional (D) histological approaches. Therefore, our objective was to develop a workflow that allows examination of the innervation of the intact mouse knee joint in 3D by employing clearing-enabled light sheet microscopy. We first surveyed existing clearing protocols (SUMIC, PEGASOS, and DISCO) to determine their ability to clear the whole mouse knee joint, and discovered that a DISCO protocol provided the optimal transparency for light sheet microscopy imaging.
View Article and Find Full Text PDFArticle Synopsis
- Osteoarthritis (OA) is a painful joint disease related to neuroimmune interactions, with macrophages in the dorsal root ganglia (DRG) contributing to OA pain in animal models.
- In experiments, researchers depleted certain macrophages in male and female mice using a specific drug to assess changes in pain behaviors and joint damage following two types of joint surgeries.
- Results showed that macrophage depletion reduced pain symptoms in both male and female mice but did not affect cartilage damage or inflammation levels, while specific types of macrophages in the DRG were significantly decreased after treatment.
Article Synopsis
- The study aimed to investigate changes in nociceptor (pain receptors) remodeling in the knee joints of mice at different stages of osteoarthritis (OA).
- Researchers used various mouse models with knee joint injuries and examined the effects over time, assessing nerve growth and joint damage.
- The results indicated that nociceptor sprouting and related pain pathways become more pronounced in early-stage OA, which may provide insights into understanding OA-related pain better.
A major barrier that hampers our understanding of the precise anatomic distribution of pain sensing nerves in and around the joint is the limited view obtained from traditional two dimensional (D) histological approaches. Therefore, our objective was to develop a workflow that allows examination of the innervation of the intact mouse knee joint in 3D by employing clearing-enabled light sheet microscopy. We first surveyed existing clearing protocols (SUMIC, PEGASOS, and DISCO) to determine their ability to clear the whole mouse knee joint, and discovered that a DISCO protocol provided the most optimal transparency for light sheet microscopy imaging.
View Article and Find Full Text PDF