Publications by authors named "N A Vaulin"
Article Synopsis
- Experimental methods in single-molecule enzymology enable scientists to analyze the unique properties and function of individual enzyme molecules during their catalytic processes.
- The study utilizes solid-state nanopores, specifically a 5 nm pore in a silicon nitride chip, to observe the performance of cytochrome P450 BM3, a model enzyme in monooxygenase systems.
- By measuring ion current changes while the enzyme catalyzes laurate hydroxylation, the research showed that the BM3 enzyme is active for up to 1500 seconds, with potential applications in developing sensitive detectors for enzyme studies.
This work demonstrates the use of a solid-state nanopore detector to monitor the activity of a single molecule of a model enzyme, horseradish peroxidase (HRP). This detector includes a measuring cell, which is divided into cis- and trans- chambers by a silicon nitride chip (SiN structure) with a nanopore of 5 nm in diameter. To entrap a single HRP molecule into the nanopore, an electrode had been placed into the cis-chamber; HRP solution was added into this chamber after application of a negative voltage.
View Article and Find Full Text PDFThe three-dimensional organization of the genome plays a central role in the regulation of cellular functions, particularly in the human brain. This review explores the intricacies of chromatin organization, highlighting the distinct structural patterns observed between neuronal and non-neuronal brain cells. We integrate findings from recent studies to elucidate the characteristics of various levels of chromatin organization, from differential compartmentalization and topologically associating domains (TADs) to chromatin loop formation.
View Article and Find Full Text PDFCurrently, nanopore-based technology for the determination of the functional activity of single enzyme molecules continues its development. The use of natural nanopores for studying single enzyme molecules is known. At that, the approach utilizing artificial solid-state nanopores is also promising but still understudied.
View Article and Find Full Text PDFUnlabelled: Data on rapid effects of statins in patients (pts) with acute coronary syndrome (ACS) are mostly from trials of atorvastatin (ATO). We hypothesized that due to high potency 10 mg of rosuvastatin (ROS) would produce same changes of lipids and inflammation markers as 40 mg ATO.
Methods: We openly randomized 53 pts (69.