Differential Expression of Local Immune Response Genes in the Vagina: Implication for the Diagnosis of Vaginal Infections.

Transcription profiles of genes of local immune response were determined in the vagina of women with bacterial vaginosis, aerobic vaginitis, and vulvovaginal candidosis for detection of the most specific immune markers for these vaginal infections. Laboratory diagnosis of the vaginal infections was performed microscopically; the inflammatory reaction in the vagina (leukorrhea) was defined as the presence of >10 white blood cells per field of view. Transcription profiles of IL1b, IL10, IL18, TNFα, TLR4, GATA3, and CD68 were determined using reverse-transcription quantitative real-time PCR.

[The vaccinal prevention of diphtheria and tetanus in children suffering from bronchial asthma and asthmatic bronchitis].

The course of the vaccinal process was studied in 69 children with bronchial asthma and asthmatic bronchitis after the injection of adsorbed diphtheria-tetanus (DT) toxoid with reduced antigen content. After immunization all these children were found to form protective levels of anti-diphtheritic and antitetanus antibodies irrespective of the severity and duration of the remission of the main disease prior to vaccination; at the same time no obstructive changes in bronchial ventilation were observed after immunization with adsorbed DT toxoid with reduced antigen content. The schemes of the preparatory medicinal treatment of patients at the period of vaccination are given with due regard for the duration of the remissions of bronchial asthma and asthmatic bronchitis.

[Vaccination of children with allergic diseases in foci of diphtheria infection].

As many as 11 children suffering from allergic diseases were vaccinated in 2 foci of diphtheritic infection. At the moment of immunization 3 children manifested a subacute course of atopic dermatitis, 4 children with bronchial asthma were vaccinated during a short-term disease remission (from 10 days to 2 months). Provided the children received combined therapy and were seen by the allergologist, the postvaccinal period ran its course without any side reactions.

[The functional diagnosis of bronchial hyperreactivity and bronchial lability in children with bronchial asthma].

During a remission, bronchial asthma children were examined for bronchial hyperreactivity and bronchial lability by means of graded physical exercise tests, and pharmacological tests with bronchoconstrictors and bronchodilators. It has been established that normal lung function seen during aa remission does not mean the lack of bronchial hyperreactivity whereas incomplete recovery of bronchial patency during bronchial asthma remission is always attended by bronchial hyperreactivity in almost all the cases. In carrying out functional tests for bronchial hyperreactivity and bronchial lability, the recording of the velocity parameters of forced expiration making it possible to define patency of not only the central but also of the peripheral bronchi appeared very informative.