[Decrease in the level of DeoR-dependent repression of the deo operon as a result of integration of foreign DNA fragments into the interoperator deoO1-deoO2 region of the Escherichia coli chromosome].

Two heterologous DNA fragments encoding the genes Kanr and cat were integrated into the regulatory region of the deo operon of Escherichia coli, located in a recombinant plasmid. As a result, plasmids pD5K and pD5C were obtained, in which the distance between the deoO1 and deoO2 operators increased from 599 bp in the deo operon of the wild type to 2019 and 1398 bp in case of integration of Kanr and cat, respectively. Then, linearized DNA of plasmids pD5K and pD5C was incorporated into the deo locus of the chromosome of E.