[Experience in realizing the Harm Reduction program in Moscow].

The data on the realization of the program of the prevention of HIV infection among intravenously injecting drug users in the South-Western administrative district of Moscow are presented. In the course of the realization of the project drawn up in accordance with this program the information package permitting the collection, analysis and transmission of the necessary information on the epidemic situation and leading risk factors in the zone of the action of the project has been compiled, and on the basis of this information the tactics and strategy of preventive measures have been worked out. The main advantage of the information system thus developed is the possibility of its use in computers of any class, in any operational system, as well as its use in Internet.

[The prevention of HIV infection in abusers of psychoactive substances as a social behavioral problem].

This research has made it possible to outline and illuminate a range of problems which should be solved in planning concrete measures for the prophylaxis of HIV infection among the users of psychoactive substances. Such measures should also include those aimed at preventing children and adolescents from getting accustomed to the use of drugs at an early age, as well as the search of ways for providing adequate and effective information on the consequences of using narcotic drugs, the prevention of passage from single cases of the tentative use of drugs to systematic narcotization, etc.

[The modelling of the genetic correction of familial hypercholesterolemia by creating cellular clones stably expressing the human low-density lipoprotein receptor].

The stable expression of human low-density lipoprotein receptor (rLDL) was studied in Chinese hamster ovary (CHO) cells transformed by the pMSVL recombinant plasmid. This plasmid contains full-length rLDL cDNA and hybrid promoter consisting of regulatory elements from early SV-40 promoter and glucocorticoid responsive enhancer of Moloney sarcoma virus. The expression of rLDL cDNA in transformed cells was demonstrated using RNA-cDNA blot hybridization, PCR analysis, and immunofluorescent analysis.