Genotoxin-producing induces tissue-specific types of DNA damage and DNA damage response outcomes.

Introduction: Typhoid toxin-expressing causes DNA damage in the intestinal mucosa , activating the DNA damage response (DDR) in the absence of inflammation. To understand whether the tissue microenvironment constrains the infection outcome, we compared the immune response and DDR patterns in the colon and liver of mice infected with a genotoxigenic strain or its isogenic control strain.

Methods: spatial transcriptomic and immunofluorescence have been used to assess DNA damage makers, activation of the DDR, innate immunity markers in a multiparametric analysis.

One-dose therapy of Candida vaginitis. I. Results of an open multicentre trial.

90 female patients suffering from Candida vaginitis were included in an open, mycologically controlled study, carried out at three gynaecological clinics, to investigate the efficacy and tolerance of one single vaginal tablet containing 500 mg clotrimazole (Canesten 1). In 40% of the patients the infection was considered to be mild and in 60% severe. In order to confirm the diagnosis and to evaluate the therapeutic effect (before application and 1 as well as 4 weeks after therapy), the following criteria were considered: (1) identification of Candida species on direct microscopic examination (wet film) and also by culture on a Nickerson medium, and (2) influence on the clinical symptoms, such as itching, burning, pathological changes of the vaginal mucosa, thrush.

Treatment off vulvovaginal candidal infection with miconazole-coated tampons.

In an evaluation of three different treatment regimens miconazole-coated tampons proved to be effective for the relief of vulvovaginal candidal infection. A mycological and clinical cure rate of about 93% was achieved in patients treated with one tampon twice daily (up to a total of 15 tampons); 61% of patients were mycologically cured with a regimen of one tampon daily for only five days. Reinfection rates were less than 4% with all three regimens.

The influence of preconceptional glucose values on the outcome of pregnancy.

A relationship between maternal diabetes and fetal malformations has been recognized for a long time, but there has been considerable difference of opinion about the size of the risk. Several defects of the pelvis and lower limbs appear to be relatively common in offspring of diabetic mothers. In experimental studies a dose relationship has been shown between some drugs (insulin-Trypan blue) and lower limb malformations.