Parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor.

A novel betaproteobacterium, designated strain Ch07(T), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in South Korea. In order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. The new strain was Gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped.

Microbacterium soli sp. nov., an alpha-glucosidase-producing bacterium isolated from soil of a ginseng field.

Five Gram-type-positive, aerobic, rod-shaped, non-motile strains of Microbacterium (DCY 17(T), Ms1, Ms2, Ms3 and Ms4) were isolated from soil from a ginseng field in Daejeon, South Korea. On the basis of 16S rRNA gene sequence similarity, these strains were shown to be related to Microbacterium esteraromaticum DSM 8609(T) (96.1 %), M.

Nocardioides humi sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.

Strain DCY24(T), a Gram-reaction-positive, aerobic, rod-shaped, motile bacterium, was isolated from soil of a ginseng field in South Korea. According to 16S rRNA gene sequence analysis, it was closely related to Nocardioides aromaticivorans DSM 15131(T) (95.1 % similarity), Nocardioides simplex KCTC 9106(T) (95.

Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.

A Gram-negative, motile bacterium, designated DCY36T, was isolated from soil of a ginseng field in South Korea and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain DCY36T belongs to genus Castellaniella in the family Alcaligenaceae of the class Betaproteobacteria. The 16S rRNA gene sequence similarities between strain DCY36T and the three recognized representatives of the genus, Castellaniella caeni Ho-11T, Castellaniella defragrans 54PinT and Castellaniella denitrificans NKNTAUT, were 98.

Digital holographic microscopy for quantitative cell dynamic evaluation during laser microsurgery.

Digital holographic microscopy allows determination of dynamic changes in the optical thickness profile of a transparent object with sub-wavelength accuracy. Here, we report a quantitative phase laser microsurgery system for evaluation of cellular/ sub-cellular dynamic changes during laser micro-dissection. The proposed method takes advantage of the precise optical manipulation by the laser microbeam and quantitative phase imaging by digital holographic microscopy with high spatial and temporal resolution.

Brevibacillus panacihumi sp. nov., a beta-glucosidase-producing bacterium.

Two Gram-positive-staining, endospore-forming, rod-shaped, motile bacteria, strains DCY35(T) and C17, were isolated from soil of a ginseng field in South Korea and were characterized in order to determine their taxonomic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the family Paenibacillaceae; strain DCY35(T) showed highest levels of similarity to strain C17 (99.9 %), Brevibacillus invocatus LMG 18962(T) (98.

Is warm temperature necessary to prevent urethral stricture in combined transurethral resection and vaporization of prostate?

Objectives: To compare the effect of warm and room temperature irrigation solution on the incidence of urethral stricture during combined transurethral resection and vaporization of the prostate (CTURVP). Urethral stricture after transurethral surgery of the prostate is a bothersome complication. Warm irrigation improves the blood flow and might decrease the incidence of urethral stricture compared with the use of room temperature irrigation, which decreases the blood flow in the urethral mucosa, resulting in ischemic injury.

Pseudoclavibacter soli sp. nov., a {beta}-glucosidase-producing bacterium.

A Gram-positive, aerobic, rod-shaped and non-motile bacterium, designated strain KP02(T), was isolated from soil from a ginseng field in Daejeon, South Korea. On the basis of 16S rRNA gene sequence similarity, this strain was shown to be related to 'Pseudoclavibacter alba' NBRC 15616 (95.5 %), 'Pseudoclavibacter faecalis' NBRC 15706 (93.

Degradation of endocrine disrupting chemicals by genetic transformants in Irpex lacteus with an inducible laccase gene of Phlebia tremellosa.

Irpex lacteus was genetically transformed using an laccase expression vector to get increased laccase producing strains. Stable integration of the vector was confirmed by PCR using the vector-specific primers, and the transformants showed increased laccase activities. When the transformants were grown with several endocrine disrupting chemicals, laccase activity of each transformant was induced up to six times higher than that of the wild type.

The role of mPer2 clock gene in glucocorticoid and feeding rhythms.

The circadian clock synchronizes the activity level of an organism to the light-dark cycle of the environment. Energy intake, as well as energy metabolism, also has a diurnal rhythm. Although the role of the clock genes in the sleep-wake cycle is well characterized, their role in the generation of the metabolic rhythms is poorly understood.

Gel-based proteomics approach for detecting low nitrogen-responsive proteins in cultivated rice species.

Nitrogen fertilization is essential for increasing rice production to meet the food demands of increasing world's population. We established an in vivo hydroponic rice seedling culture system to investigate physio-biochemical/molecular responses of various rice japonica and indica cultivars to low nitrogen (N). Three-week-old seedlings grown in Yoshida's nutrient solution manifested stable and reproducible symptoms, such as reduced shoot growth and length under low N.

Complete bladder gangrene caused by bilateral hypogastric artery ligation during laparoscopic radical hysterectomy.

Ischemic complications are extremely rare after radical pelvic surgery because of the collateral blood supply in the pelvis. We report a case of complete bladder gangrene 3 weeks after a laparoscopic radical hysterectomy with bilateral hypogastric artery ligation in a 70-year-old woman with cervical cancer.

Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field.

A Gram-positive, non-motile, pale-yellow, short rod-shaped bacterium, strain DCY26(T), was isolated from soil of a ginseng field in South Korea and was investigated to determine its taxonomic position. The organism grew optimally at 30-37 degrees C. The G+C content of its DNA was 65.

Unusual presentation of right-side accessory spleen mimicking a retroperitoneal tumor.

An unusual case of right-side retroperitoneal accessory spleen is presented. A 68-year-old man visited our hospital for the management of incidentally detected retroperitoneal mass. The computed tomography scan of the abdomen revealed the presence of a retroperitoneal tumor (4.

Aeromicrobium ginsengisoli sp. nov., isolated from a ginseng field.

Strain Gsoil 098(T), a Gram-positive, non-spore-forming, non-motile coccus, was isolated from soil from a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Gsoil 098(T) belongs to the family Nocardioidaceae, and the highest degrees of sequence similarity were found with Aeromicrobium marinum T2(T) (99.0%), A.

Digital holography of total internal reflection.

We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.

Clinical and radiological results after modified distal metatarsal osteotomy for hallux valgus.

Background: The chevron osteotomy is an acceptable method for correction of mild and moderate hallux valgus, but can result in instability at the osteotomy site. The purpose of this study was to present clinical and radiological results with our modified technique of osteotomy.

Materials And Methods: We performed a modified technique of distal osteotomy of the first metatarsal on 77 feet of 46 patients with symptomatic hallux valgus; followed up for an average of 52 months.

Removal of estrogenic activity from endocrine-disrupting chemicals by purified laccase of Phlebia tremellosa.

A white-rot basidiomycete, Phlebia tremellosa, produced a laccase that showed increased activity during degradation of phthalates. A laccase was purified through the ion exchange chromatography and preparative gel electrophoresis, and the estimated molecular weight was 75 kDa. The optimum pH and temperature of the purified laccase was pH 4.

Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses.

Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties.

Paenibacillus ginsengihumi sp. nov., a bacterium isolated from soil in a ginseng field.

Strain DCY16T, a Gram-positive, spore-forming, rod-shaped, motile bacterium, was isolated from soil and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY16T belonged to the genus Paenibacillus; highest sequence similarities were with Paenibacillus validus JCM 9077T (94.4 %), P.

Sanguibacter soli sp. nov., isolated from soil of a ginseng field.

A Gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain DCY22(T), was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY22(T) belonged within the family Sanguibacteraceae, and highest levels of sequence similarity were found with Sanguibacter marinus 1-19(T) (96.8 %), Sanguibacter suarezii ST-26(T) (96.

Unusual complication of urethral catheterization: a case report.

We report an unusual complication caused by urethral catheterization. During a routine urethral catheter change in a 38-yr-old woman, a 14-Fr Foley catheter was accidentally placed into the right ureter through the ureteral orifice. The position of the catheter was confirmed by retrograde urogram through urethral catheter.

Prevention of urethral stricture in insertion of an inflatable penile prosthesis.

Objective: The aim of this study was to find the mechanism that causes urethral stricture with a view to preventing its occurrence after implantation of a three-piece inflatable penile prosthesis.

Methods: When implanting a three-piece inflatable penile prosthesis, we draw two longitudinal lines parallel to the long axis of the proximal cylinder and the tubing leading from each cylinder into the cavernosal space, to prevent 360 degree rotation of the cylinder.

Results: From September 1993 to February 2007, a total 86 three-piece penile prosthesis implantations were carried out in 70 patients.

Microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.

Strain Gsoil 259(T), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain Gsoil 259(T) belonged to the genus Microbacterium and was closely related to Microbacterium arborescens IFO 3750(T) (98.