Involvement of Ca3.2 T-type Ca channels and cystathionine-β-synthase in colitis-related visceral hypersensitivity in mice.

We tested the hypothesis that Ca3.2 T-type Ca channels, which can be rebooted by sulfides from Zn inhibition under physiological conditions, and sulfide-generating enzymes including cystathionine-β-synthase (CBS) would participate in the colitis-related visceral pain in mice treated with 2,4,6-trinitrobenzene sulfonic acid (TNBS). The visceral hypersensitivity following TNBS-induced colitis was abolished by an inhibitor or genetic deletion of Ca3.