Use of the Er,Cr:YSGG laser as an adjuvant in root canal system disinfection.

Background: This study investigated the antibacterial efficacy and cleaning capacity of the Er,Cr:YSGG laser in root canal disinfection.

Material And Methods: Fifteen teeth were inoculated with an biofilm and assigned to three groups as follows: Group 1 (control: biofilm only), Group 2 (Ultrasound [US] + 5.25% NaOCl), and Group 3 (Er, Cr at 1.

Food Insecurity and Dental Caries in Rural Mexican Populations.

Objective: Food insecurity (FI) is a priority for government and health organizations. Over 95% of the world's population has a carious lesion or will develop one before death. This study evaluated the association between FI and oral health in two rural communities in Chiapas, Mexico.

Effect of Culture Conditions of Endophytic Fungi on Yield and Anticancer and Antioxidant Activities.

Culture conditions affect the production of secondary metabolites in endophytic fungi. Therefore, the aim of the present study was to evaluate the yield and anticancer and antioxidant activity of endophytic fungi extracts from the cactus , under different culture conditions. The strains , , and sp.

Antibacterial efficacy of a pH-neutral electrolyzed super-oxidized solution for nonsurgical periodontal treatment.

Objective: Mouthwash is effective in maintaining oral hygiene in patients; however, there is concern that it may adversely affect human oral mucosa. We evaluated a pH-neutral electrolyzed super-oxidized solution (ESS, tradename OxOral®) combined with dental scaling in periodontitis patients. This longitudinal study was conducted with 34 patients divided into three groups.

Cytotoxicity of Peruvian propolis and on human gingival fibroblasts, PBMCs and HeLa cells.

It is indisputable that every day it is demonstrated that natural products present diverse therapeutic benefits, which has boosted their incorporation within various products for clinical use. However, this must be accompanied by knowledge of their effect on cell lines to ensure their use is safe. The objective of this study was to evaluate the cytotoxic effect of two ethanolic extracts based on Peruvian natural products, on three human cell lines.

Antimicrobial effectiveness of root canal sealers against .

We evaluated four root canal sealers to determine their antimicrobial effectiveness against . The direct contact test was used to measure the effectiveness of the study materials and close contact between bacteria on the kinetics of bacterial growth. The agar diffusion test (ADT) was also performed for comparison.

SSEA-4 positive dental pulp stem cells from deciduous teeth and their induction to neural precursor cells.

Background: Stage-specific embryonic antigen-4 (SSEA-4) is a marker for the identification of multipotent embryonic cells. It is also positive in neuroepithelial cells, precursor neural cells (NPC), and human dental pulp cells. The aim of this study was to evaluate the potential morphodifferentiation and histodifferentiation to NPC of SSEA-4 positive stem cells from human exfoliated deciduous teeth (SHED).

Antimicrobial Effect of Calcium Hydroxide Combined with Electrolyzed Superoxidized Solution at Neutral pH on Growth.

Objective: To evaluate the effect of the combination of calcium hydroxide (Ca(OH)) and a novel electrolyzed superoxidized solution at neutral pH, known as OxOral® on growth in root canals.

Methods: Sixty human teeth were used, from which root canals were infected and randomly divided into the following treatment groups: saline solution, saline solution plus Ca(OH), OxOral®, and OxOral® plus Ca(OH).

Results: A permanent reduction in bacterial growth was observed at days 1, 6, 12, and 18 after OxOral® plus Ca(OH) treatment from 4.

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against .

The presence of in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic success rates, where the use of mouthwashes with anti- activity would help to achieve it. The aim was to evaluate the antimicrobial activity of OxOral® mouthwash against and its effect on biofilm formation.

Molecular detection of based on the presence of and virulence genes in dental plaque from patients with periodontitis.

Background/purpose: () infection is the most common in the world and is associated with various gastrointestinal pathologies, including chronic gastritis, peptic ulcers, and gastric cancer. The prevalence is associated with socioeconomic conditions, with this infection being more common in developing countries than in developed countries. The presence and permanence of in the oral cavity has been reported, but its role is controversial.

Immunoinformatics Approach to Design a Novel Epitope-Based Oral Vaccine Against .

is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the increase in resistance to antibiotics used in the anti- therapy, the development of an effective vaccine is an alternative of great interest, which remains a challenge.

Immunization with a Synthetic Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection.

is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against , an appropriate vaccine design targeting conserved/essential genes must be identified. In the present study, a 50-52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against infection.

Dental iron precipitates in patients with Type 2 diabetes.

Diabetes mellitus (DM) is a frequent worldwide disease. There are currently more than 46 million people who suffer this disease in North America and the Caribbean. The objective of this study was to determine if there is an association between DM and the presence of iron precipitates (Fe) in dental structure.

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of -Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

Αlpha-solanine (-solanine) is a glycoalkaloid present in potato . It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of -solanine on cell physiology.

In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin.

Helicobacter pylori is an infectious agent commonly associated with gastrointestinal diseases. The use of probiotics to treat this infection has been documented, however, their potential antimicrobial metabolites have not yet been investigated. In the present study, the effect of reuterin produced by Lactobacillus reuteri on H.

Miconia sp. Increases mRNA Levels of PPAR Gamma and Inhibits Alpha Amylase and Alpha Glucosidase.

Diabetes mellitus is a public health problem worldwide. For this reason, ethanolic extract of Miconia sp. from Oaxaca, Mexico, was selected in search of an alternative against this disease.

Antimicrobial properties and dental pulp stem cell cytotoxicity using carboxymethyl cellulose-silver nanoparticles deposited on titanium plates.

To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of and . The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.

Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro.

Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs).

Material And Methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry.

Antimicrobial Effect of Lactobacillus reuteri on Cariogenic Bacteria Streptococcus gordonii, Streptococcus mutans, and Periodontal Diseases Actinomyces naeslundii and Tannerella forsythia.

Lactic acid bacteria (LAB) are well known for their beneficial effects on human health in the intestine and immune system; however, there are few studies on the impact they can generate in oral health. The aim of this study was to test and compare in vitro antimicrobial activity of L. reuteri on pathogenic bacteria involved in the formation of dental caries: S.