Effect of Dilution Media and Incubation Time on Motility, Kinetic and Morphometric Parameters of Canine Spermatozoa Using CASA System.

The present study aimed to evaluate the effect of two dilution media and five incubation times at 37°C on the kinetic and morphometric parameters of canine spermatozoa (spz) using computer-assisted sperm analysis (CASA). To do so, 14 ejaculates were collected from six dogs, and initially assessed (microscopy for motility and photometer for concentration). Each ejaculate was divided into two aliquots and diluted in two different solutions (PBS 'phosphate-buffered saline' and a commercial buffer solution 'EBB: Easy Buffer B') at a concentration of 25 million spz/mL then incubated at 37°C for five different times (T0, 10, 20, 30 and 40 min after collection) before being analysed by Hamilton-Thorne IVOS II CASA system.