[Effect of N-stearoylethanolamine on the DNA fragmentation intensity in tumour and extratumoral tissues of the human adrenal cortex].

The effect of different concentrations of N-stearoylethanolamine (NSE 18:0) on fragmentation of DNA in the tumoural and extratumour tissues of the adrenal glands in vitro was studied. In this work the following types of tissue were investigated: extratumoural tissue from patients with hormonally active tumours, benign tumour tissue (hormonally active and hormonally inactive), tissue of malignant tumours and hyperplasic tissue of the adrenal glands (Itsenko-Cushing disease). It has been established that the NSE increases the intensity of DNA fragmentation only in the tissue of hormonally inactive tumours.

[The effect of potassium different concentrations on mRNA expression of protein StAR and cytochrome p-450(SCC) in human adrenocortical tissue].

The effect of different potassium concentrations on changes in steroidogenic acute regulatory protein (StAR) and cytochrome P-450(SCC) in human adrenal cortex tissue was studied. A rise in K+ concentration in incubation medium to 8.5 mM caused an increase in StAR and cytochrome P-450(SCC) mRNA level in adrenocorticocytes by 70 and 76%, respectively, compared with control.

[The modelling of N-stearoylethanolamine effect of 17beta-estradiol in the organism of male rats].

The influence of N-stearoylethanolamine (NSE) on total 11-hydroxycorticosteroids (11-HCS) and testosterone level in the blood of male rats in normal conditions and under the action of 17beta-estradiol (400 mkg/kg of body weight during 3 days) was studied. It was shown that NSE administration per os (50 mg/kg of body weight during 7 days) to intact animals did not change the level of 11-HCS and of testosterone. The administration of NSE to estrogenized male rats decreased the elevated level of 11-HCS and normalized the amount of testosterone in blood.

[Effect of 17beta-estradiol on mRNA expression of Bax proapoptotic protein and DNA fragmentation level in the human adrenal cortex].

The effect of 17beta-estradiol on the change of proapoptotic protein Bax mRNA level and DNA laddering in human adrenal cortex tissue was studied. The adding of 17beta-estradiol to incubation medium caused a decrease of the proapoptotic protein Bax mRNA level in adrenocorticocytes by 22% in comparison with control. Hormone influence decreases the DNA fragmentation by 50%.

[The influence of protein kinases ERK, JNK and nuclear transcriptional factor c-JUN on corticotropin signal transduction in adrenocortical cells].

The estradiol effect on the expression level of ERK1/2, JNK, p38 mitogen-activated protein kinases, c-Jun and c-Fos transcription factors in the adrenal cortex of male rats was studied. It was shown that a single corticotropin injection caused significant changes of the ERK1/2 protein kinases levels in the adrenal cortex. This index was 1.

[Participation of cyclic nucleotides, protein kinase A and C in realization of n-acylethanolamine action in human adrenocortical cells].

The messenger mechanisms mediating N-acylethanolamines (NAE) regulatory signals in the adrenal cortex were studied. An analysis of the mechanisms of realization of NAE effects in the post-operation human adrenal cortex was carried out in vitro. Influence of NAE mix on cAMP and cGMP level, protein kinase A and C activity in sub-cellular fraction of adrenocorticocytes and homogenates of conditionally normal adrenal cortex tissues was investigated.

[Effect of adrenocorticotropic hormone on the caspase-3 level and DNA fragmentation in hyperplasia tissue of human adrenals].

The effect of corticotropin and inhibitor of protein kinase C, chelerythrine chloride, on the change of caspase-3 level and on the rate of DNA laddering in hyperplasia adrenal cortex tissue of patient with Cushing's syndrome was studied. It was established that ACTH caused significant antiapoptotic effect in the human adrenal cortex. The adding of ACTH to incubation medium caused a sharp decrease of the caspase-3 level in adrenocorticocytes.

[Effects of estradiol on synthesis of corticosteroids, protein kinase A and C activity in the adrenal cortex of intact and orchiectomized rats].

Estradiol treatment produced significant increase of total 11-hydroxycorticosteroids level in the blood of intact and castrated rats. Activity of protein kinase A increased in the cytosol and membrane fraction of adrenocorticocytes of intact and orchiectomized rats after estradiol influence. Activity of protein kinase C significantly raised in the cytosol and membrane fraction of adrenocortical cells in all investigated groups.

[The role of phosphoinositides, protein kinase C and protein kinase A in the K+ regulatory signal transduction in human adrenocortical cells].

The messenger mechanisms mediating K+ regulatory signals in human adrenocorticocytes were studied. It was shown that potassium ions initiated decay of polyphosphoinositides to inositolphosphates and obviously diacylglycerol. The latter compounds activate protein kinase C as affected by different agonists.

[ACTH activates in vitro protein kinases A and C in human adrenal cortex].

The activation of protein kinases A and C by corticotropin in the human adrenal cortex was analyzed. ACTH influence in vitro significantly increase protein kinase A activity, total protein kinase C activity in the membrane fraction of adrenocorticocytes and 11-hydroxycorticosteroids production of adrenal cortex tissue. Either cAMP-dependent protein kinase A or protein kinase C are included to postreceptoral messenger mechanism, that mediates ACTH action in adrenocortical cells.