Biologically active compounds containing sulfhydryl groups (RSHs: N-acetyl-l-cysteine, d-penicillamine, glutathione and acetylthiocholine chloride) were used to develop a luminometric method for their quantification. The title substrate capable of chemiluminescence (CL) was isolated in a highly pure state as a chloride salt (99.9% using RP-HPLC) and identified using mass spectrometry (ESI Q-TOF) and H NMR spectroscopy.
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