Resveratrol alleviates salivary gland dysfunction induced by ovariectomy in rats.

Resveratrol (Res), found abundant in many medicinal plants, exerts multiple biological functions in the body, including anti-inflammatory, antioxidant, and anti-aging properties. Xerostomia is a major symptom of salivary gland dysfunction in menopausal women, which significantly compromises the quality of life. Here, we investigated the effect of Res on estrogen deficiency-induced salivary gland dysfunction in rats.

Dietary Nitrate Protects Against Skin Flap Ischemia-Reperfusion Injury in Rats Modulation of Antioxidative Action and Reduction of Inflammatory Responses.

Dietary nitrate, found abundant in green vegetables, can be absorbed into the blood and be converted to nitric oxide (NO) in the body. Dietary nitrate has been proved to have many positive physiological functions in the body. Here, we evaluated the therapeutic effects of dietary nitrate on skin flap recovery following ischemia reperfusion (IR).

Dietary nitrate protects skin flap against ischemia injury in rats via enhancing blood perfusion.

Insufficient blood supply is associated with high levels of necrosis in reconstructive surgery. Restoring blood flow to undersupplied ischemic tissue is the most important impact factor determining skin flap viability. Dietary nitrate, a significant source of nitric oxide, has multiple physiological functions, including regulator of blood flow, angiogenesis, and vasodilatation.

Clinical significance of cytokeratin in the cervical lymph nodes of patients with mandibular gingival squamous cell carcinoma.

The present study aims to analyze the expression of broad spectrum cytokeratin in the cervical lymph nodes of patients with mandibular gingival squamous cell carcinoma and explore the metastasis of mandibular gingival squamous cell carcinoma in cervical lymph nodes. This study included 42 patients with mandibular gingival squamous cell carcinoma, which was staged according to the clinical staging criteria by International Union Against Cancer 2002 (UICC) and the Level staging method of cervical lymph node by American Academy of Otolaryngology-Head and Neck Surgery 1991. Monoclonal mouse anti-human cytokeratin (AE1/AE3) antibody was used in immunohistochemical examination and hematoxylin and eosin (H&E) staining.

[Expression and clinical significance of the genes of Wnt signaling pathway in keratocystic odontogenic tumor of the jaw bones].

Purpose: To study the role of genes of Wnt signaling pathway in keratocystic odontogenic tumor (KCOT) of the jaw bones.

Methods: Fresh specimens of KCOT and the same patient 's normal oral mucosa were obtained. Then RNA was extracted.

[The effect evaluation of suction drainage to prevent fistula after superficial parotidectomy].

Purpose: To evaluate the clinical value of suction drainage to prevent fistula after parotidectomy, and seek the best opportunity to remove the drainage according to the draining output and duration.

Methods: One hundred and ninety-four patients with parotid diseases after superficial parotidectomy were assigned into pressure dressing group and suction drainage group. Pressure dressing was used after suction drainage tube was removed in the pressure group, while suction drainage tube was fixed through the process in the suction group.

Gene mutations in the D-loop region of mitochondrial DNA in oral squamous cell carcinoma.

The present study aimed to investigate gene mutations in the displacement‑loop (D‑loop) region of mitochondrial DNA (mtDNA) in patients with oral squamous cell carcinoma (OSCC) in order to examine the role of gene mutation in mtDNA in OSCC tumorigenesis. mtDNA was obtained from cancer tissues, paracancerous tissues and normal mucosal tissues of thirty patients with OSCC. The D‑loop region of the mtDNA was amplified using polymerase chain reaction, sequenced and then analyzed by Chromas software and BLAST to identify the mutation sites.

[Cytokeratin expression in cervical lymph nodes of patients with mandibular gingival squamous cell carcinoma].

Objective: To examine the cytokeratin expression in cervical lymph nodes of patients with mandibular gingival squamous cell carcinoma and its clinical significance.

Methods: The data of 42 cases with mandibular gingival squamous cell carcinoma after operation from July 2009 to December 2012 were included. Forty-two patients (male = 27, formale = 15) were included, with a mean age of 54.

[Evaluation of osteogenesis of alveolar bone graft by cone beam CT].

Objective: To evaluate the osteogenesis of alveolar bone graft (ABG) in patients with alveolar cleft by cone beam CT (CBCT).

Methods: ABG surgery was performed in 20 patients with unilateral complete alveolar cleft. The patients were followed up for 3 and 6 months after surgery and the osteogenesis of the bone graft was evaluated by CBCT.

[Screening of the gene mutation in D-loop region of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the gene mutation in D-loop region of mitochondrial DNA (mtDNA) in oral squamous cell carcinoma (OSCC) tissue and to explore the role of the gene mutation in D-loop region in the OSCC tumorigenesis.

Methods: mtDNA was obtained from cancer, paracancerous and normal mucosa tissues of thirty patients with OSCC. The D-loop regions of mtDNA were amplified with PCR, sequencing and then analyzed by Chromas software and BLAST to identify the mutation site.

[Use of "gelatamp" colloidal silver gelatin sponge to prevent dry socket after extracting mandibular impacted teeth].

Purpose: To study the clinical effect of "gelatamp" colloidal silver gelatin sponge on preventing dry socket after extracting the mandibular impacted teeth.

Methods: 1350 teeth extracted from 976 patients who needed to extract the mandibular impacted teeth were divided into group A, group B and group C randomly. "Gelatamp" colloidal silver gelatin sponge was implanted into alveolar socket after teeth extraction in group A, with absorbable gelatin sponge was implanted into alveolar socket in group B and nothing was implanted into alveolar socket in group C.

Dental functional status with and without tooth replacement in a Chinese adult population.

The objective of this study is to investigate the prevalence of missing teeth and prosthodontic replacements in a Chinese adult population using a hierarchical dental functional classification system. A total of 1,462 dentate subjects over 40 years from Shandong Province, China were included and categorized in the functional classification system with and without tooth replacements. Depending on replacements, subjects could be reclassified (promoted) to categories reflecting higher functionality.

[The mutations of germline succinate dehydrogrnase subunit B (SDHB) in sporadic paragangliomas].

Purpose: The purpose of this study was to investigate the somatic mutations of human mitochondria succinate dehydrogenase subunit B (SDHB) in sporadic paragangliomas.

Methods: Eight exons of SDHB gene in 8 sporadic paragangliomas cases were amplified by PCR and sequenced, respectively. The sequences were analyzed to find mutations compared with human homology sequence in Genebank and SNP database.

[The mutations of the D-loop hypervariable region II and hypervariable region III of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the frequency of mitochondrial DNA (mtDNA) D-loop hypervariable region II (HVR II) and hypervariable region III (HVR III) mutations in oral squamous cell carcinoma (OSCC) and their correlation to provide the new targets for the prevention and treatment of OSCC.

Methods: The D-loop HVR II and HVR III regions of mtDNA in seven cases with OSCC tissues, matched with paracancerous tissues and normal mucosa tissues from the same case, were amplified by polymerase chain raction (PCR), then were detected by direct sequencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

Results: 82 (56 species) nucleotide changes, with 51(26 species) nucleotide polymorphism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

[The value of single photon emission computed tomography-computed tomography in diagnosis of carcinoma invasion of mandible].

Objective: To investigate the value of single photon emission computed tomography(SPECT)-computed tomography(CT) in diagnosis of oral carcinoma invasion to mandible.

Methods: Thirty-four patients with oral carcinoma invasion to mandible were divided into two groups, group A (surrounding invasion) and group B (central invasion). The edge of the invasion was evaluated by SPECT-CT, CT and pathological examination.

[A comparison between CT and SPECT in detecting mandibular invasion by lower gingival squamous cell carcinoma].

Purpose: The purpose of this study is to compare the value of CT and SPECT in diagnosis of lower gingival carcinoma invading the mandible.

Methods: From February 2002 to October 2006, twenty-one patients with lower gingival squamous cell carcinoma were enrolled.The data of CT and SPECT were studied,and compared with histopathological findings.

[Clinical biological behavior and treatment strategy of hemangioma in oral and maxillofacial region].

Hemangioma can not be prevented until nowadays. Early identification and definite diagnosis are necessary to achieve the best outcome. The clinical biological behaviors of hemangiomas in oral and maxillofacial region are completely investigated and deeply analyzed in this paper.

[Early laser intervention of hemangioma in facial and neck regions of infant].

Objective: The study was to evaluate the method of early laser intervention of hemangioma in facial and neck regions of infant.

Methods: Between January 1999 and December 2006, twelve patients, aged 6 days to 3 months, with cutaneous hemangioma in facial and neck regions, were treated with laser, eight cases with Nd:YAG laser therapy and four cases with Venus laser therapy. Four cases with hemangioma in facial and neck regions of infant treated with oral corticosteroid were as control.

Multiple orofacial malformations in a boy who was conceived by intracytoplasmic sperm injection.

Intracytoplasmic sperm injection (ICSI) and other assisted reproductive technologies (ART) are effective treatments for infertility and are widely used at infertility clinics. Although ICSI is generally considered safe, some studies have suggested an excess occurrence of major birth defects resulting from the procedure. Here, we present a rare case of a boy with multiple orofacial malformations who was conceived by ICSI.

[Early treatment of middle ear disease in cleft palate infants].

Objective: To explore the effect of myringotomy with insertion of tube and tympanocentesis on alleviating secretory otitis media (SOM) and hearing loss in cleft palate infants.

Methods: Nineteen cleft lip and palate infants with SOM and hearing loss (38 ears) were treated with myringotomy with insertion of ventilation tube at the same time of repair of the cleft lip, who were performed averagely at 6.9 months of age.

[Osteoma in the soft palate: report of one case and review of the literature].

Osteoma is a benign tumor, which is composed of mature differentiated bone tissue and derived from osteoplast in periosteum endothecium. Osteomas is common in the bone in clinic, however, osteoma in the soft tissue is distinctly rare. Clinically, the lesion presents as hard, slowly growing mass with clear border.

[Resection of carotid body tumor under interrupted block of common carotid artery].

7 patients with carotid body tumor (CBT) were evaluated by DSA preoperatively. Carotid artery (CA) blocking test was carried out under local anesthesia before operation. With CA blocked temporarily, tumors were resected under general anesthesia after the test was normal.

[Using fenestration and decompression technique to treat enormous odontogenic keratocyst].

3 cases of enormous odontogenic keratocyst were treated with the technique of fenestration and decompression other than removal of jaws. After a follow-up period of two years, radiographic assessment showed that the image of keratocyst disappeared basically, neogenetic bone had been found and the defect of jaws had been restored. The teeth had no dysfunction, the lower lip had no numbness and the keratocyst epithelium was modulated histologically to mucosa after decompression.