Proteomic perspectives on thermotolerant microbes: an updated review.

Introduction: Thermotolerant microbes are a group of microorganisms that survive in elevated temperatures. The thermotolerant microbes, which are found in geothermal heat zones, grow at temperatures of or above 45°C. The proteins present in such microbes are optimally active at these elevated temperatures.

Impact of COVID-19 pandemic on pediatric cardiac services in India.

Background: COVID-19 pandemic has disrupted pediatric cardiac services across the globe. Limited data are available on the impact of COVID.19 on pediatric cardiac care in India.

Fabrication and characterization of Spinacia oleracea extract incorporated alginate/carboxymethyl cellulose microporous scaffold for bone tissue engineering.

In recent years, plant based scaffold due to its inherent properties such as mechanical stability, renewability, easy mass production, inexpensiveness, biocompatibility and biodegradability with low toxic effects have received much attention in the field of bone tissue engineering. Design of good tissue compatible plant based polymer scaffold plays a vital role in biomedicine, nanomedicine and in various tissue engineering applications. The present study focused on the fabrication of a novel herbal scaffold using the medicinal plants Spinacia oleracea (SO) and Cissus quadrangularis (CQ) extracts incorporated with Alginate (Alg), Carboxy Methyl Cellulose (CMC) by lyophilization method.

Pectin extraction from Helianthus annuus (sunflower) heads using RSM and ANN modelling by a genetic algorithm approach.

In this work, Response Surface Methodology (RSM) and Artificial Neural Network coupled with genetic algorithm (ANN-GA) have been used to develop a model and optimise the conditions for the extraction of pectin from sunflower heads. Input parameters were extraction time (10-20 min), temperature (40-60 °C), frequency (30-60 Hz), solid/liquid ratio (S/L) (1:20-1:40 g/mL) while pectin yield (PY%) was the output. Results showed that ANN-GA had a higher prediction efficiency than RSM.

Rapid snare sliding technique: An easy approach to difficult percutaneous transmitral commissurotomy.

Rheumatic heart disease (RHD) is a major health hazard in most developing countries as well as sporadically in developed economies with high prevalence rates. Mitral stenosis is among the important morbid valvular conditions accounting for 25% of RHD cases. Percutaneous transmitral commissurotomy (PTMC) using Inoue balloon is the standard treatment for severe mitral stenosis with favorable Wilkins score.

Carboxymethylation of pectin: Optimization, characterization and in-vitro drug release studies.

The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study. PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time). Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).

A sequential pretreatment of lignocelluloses in bamboo biomass to fermentable sugars by acid/enzymatic hydrolysis.

A sequential pretreatment method for hydrolyzing rigid hemicelluloses and cellulose content in the bamboo biomass was investigated in this study. The effects of different parameters, such as nature of biomass, type of acid, acid and biomass concentration, were studied. Under the optimum condition of 5% (v/v) HCl-treated biomass and biomass concentration (8%, w/v), the maximum yield of sugar (619 mg/g of biomass) was obtained.

Xanthan gum production using jackfruit-seed-powder-based medium: optimization and characterization.

Xanthan gum (XG) production by Xanthomonas campestris NCIM 2961 using jackfruit seed powder (JSP) as a novel substrate was reported. Central composite design (CCD) of response surface method (RSM) was used to evaluate the linear and interaction effects of five medium variables (JSP, peptone, citric acid, KHPO and KHPO) for XG production. Maximum XG production (51.

Pectin from muskmelon (Cucumis melo var. reticulatus) peels: extraction optimization and physicochemical properties.

Pectin derived from plant waste sources is currently focused as an economical and eco-friendly approach. Optimization of pectin extraction from muskmelon peel by response surface methodology (RSM) was investigated in this study. Box-Behnken Design (BBD) was used to identify the optimal level of the extraction variables such as time, pH and temperature.

Incidental Ventricular Septal Defect (VSD) in the Donor of a Live Donor Liver Transplant: Tackle and Proceed.

Live Donor Liver Transplantation (LDLT) is an act of selflessness on the part of the donor who is subjected to a major hepatectomy. Ensuring safety and long-term well being of the donor is of utmost priority. We describe a 21-year-old otherwise healthy donor with perimembranous Ventricular Septal Defect (VSD) who successfully underwent donor hepatectomy after closure of the VSD.

Microbial abatement of toluene using Aspergillus niger in upflow bioreactor.

Microbial abatement of toluene using Aspergillus niger in coir packed upflow bioreactor was investigated in this study. Toluene degrading microbes were isolated from municipal sewage effluent and identified by 16s rRNA sequencing method. The microbes were cultured in 2% (v/v) toluene input per day, which exhibited 95% removal efficiency with the kinetic correction value (R) of 0.

Phytochemical composition, GC-MS analysis, in vitro antioxidant and antibacterial potential of clove flower bud (Eugenia caryophyllus) methanolic extract.

Plant derived pharmacologically active compounds have gained importance in food and pharmaceutical industries. The aim of the present study is to identify and study the antioxidant, antimicrobial properties of the phytochemicals present in the crude extract of Eugenia caryophyllus flower buds. The antioxidant activity of the methanol, acetone and chloroform extract was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.

Biodegradation of toluene vapor in coir based upflow packed bed reactor by Trichoderma asperellum isolate.

In the present study, a new biofiltration system involving a selective microbial strain isolated from aerated municipal sewage water attached with coir as packing material was developed for toluene degradation. The selected fungal isolate was identified as Trichoderma asperellum by 16S ribosomal RNA (16S rRNA) sequencing method, and pylogenetic tree was constructed using BLASTn search. Effect of various factors on growth and toluene degradation by newly isolated T.

Statistical optimization of process parameters for exopolysaccharide production by Aureobasidium pullulans using sweet potato based medium.

Statistical experimental designs were applied to optimize the fermentation medium for exopolysaccharide (EPS) production. Plackett-Burman design was applied to identify the significance of seven medium variables, in which sweet potato and yeast extract were found to be the significant variables for EPS production. Central composite design was applied to evaluate the optimum condition of the selected variables.

Testicular swelling with pneumonia and septicaemia: a rare presentation of right-sided endocarditis.

As far as we are aware, right-sided bacterial endocarditis has not previously been described as presenting with systemic illness and testicular swelling. We report a teenager who presented with this unusual combination as a consequence of right-sided endocarditis. He presented with high fever, with chills and rigor, along with painful enlargement of the left testicle, a productive cough with progressive breathlessness, and joint pains.