Ranking Technologies of Additive Manufacturing of Removable Complete Dentures by the Results of Their Mechanical Testing.

In this study, a methodology was developed for ranking manufacturing technologies of removable complete dentures (RCDs) according to the results of their full-scale mechanical tests. The actuality of the study is motivated by establishing the advantages and drawbacks of 3D-printed RCDs in contrast with ones manufactured via an analog protocol. The RCDs were fabricated via four technological routes that included various combinations of subtractive technologies (hot polymerization/HP and CAD/CAM milling) and additive manufacturing (digital light processing/DLP) ones and the installation of commercially available cosmetic denture teeth (DT).

Reconstruction of the Nose After Unilateral Cheilouranoplasty.

Congenital clefts of the maxillofacial area still remain of current interest in reconstructive facial surgery. While their frequency grows up, the issues of effective surgical primary and secondary interventions are not completely solved yet. The article presents the main problems associated with the elimination of the deformations and our modifications of methods for their correction.

Anti-Bacterial and Anti-Inflammatory Effects of Toothpaste with Swiss Medicinal Herbs towards Patients Suffering from Gingivitis and Initial Stage of Periodontitis: from Clinical Efficacy to Mechanisms.

Objective: To distinguish clinical effects and mechanisms of sodium monofluorophosphate plus xylitol and herbal extracts of Swiss medicinal plants (Chamomilla recutita, Arnica montana, Echinacea purpurea, and Salvia officinalis).

Materials And Methods: A 2-month-long comparative clinical study of toothpaste containing 1450 ppm sodium monofluorophosphate and xylitol (control, 15 patients) and toothpaste additionally containing extracts of the medicinal herbs (experiment, 35 patients) was performed on patients with gingivitis and the initial stage of periodontitis. Clinical indices of gingivitis/periodontitis were quantified by Loe & Silness's, CPITN, OHI-S, and PMA indexes.

Effects of Standardised Fermented Papaya Gel on Clinical Symptoms, Inflammatory Cytokines, and Nitric Oxide Metabolites in Patients with Chronic Periodontitis: An Open Randomised Clinical Study.

The clinical efficacy of topical administration of standardised fermented papaya gel (SFPG), known to have antioxidant and anti-inflammatory properties, versus conventional therapy was evaluated in a group of 84 patients with moderate-to-severe periodontitis, randomly assigned to control group (n = 45) undergoing traditional pharmacologic/surgical protocols or to experimental group (n = 39), additionally treated with intragingival pocket SFPG (7 g) applications (15 min daily for 10 days). Patients undergoing SFPG treatment showed significant (P < 0.05), durable improvement of three major clinical indices of disease severity: reduced bleeding (day 7), plaque and gingival conditions (day 14), and consistent gingival pocket depth reduction (day 45).

Characterization of water-soluble complexes of polyacrylic acid with alpha-Amylase from Aspergillus oryzae.

Polycomplex formation of alpha-Amylase from Aspergillus oryzae (TAKA) with polyacrylic acid (PAA) was studied by pH titration, fluorescence, and high performance liquid chromatography (HPLC) methods in water solutions. According to the our results, the complex formation and its solubility were depended on nature of enzyme and the pH of solutions. Both of them correlates isoelectric points (PI).

[Concentration of proinflammatory and antiinflammatory cytokines in blood and wound of patients with odontogenic phlegmons].

Levels of IL-1beta, IL-6, TNFalpha, IL-10 and TGF-beta in blood and wound of 56 patients with odontogenic phlegmons in dynamic of infection process were expected. In accordance with known facts production of proinflammatory cytokines (IL-1beta, IL-6, TNFalpha in blood in acute phase of the inflammation process was much more intensive than in normal conditions. Increased proinflammatory cytokines generation led to significant raising of IL-10 concentration, but elevation of its level in blood was only at the 10-14 the days of therapy.

Novel polypeptide-comprising biopolymer systems.

Covalent bioconjugation between anionic polyelecrolytes and polypeptide antigens chemically synthesized by solid-phase chemistry, were studied in hydrated reversed micelle systems. The epitops of Foot-and-Mouse disease virus VP1 protein (40--60 and 135--160 residues) were used as polypeptide antigens. The polypeptide-comprising Biopolymer Systems were obtained by two methods: 1) Inclusion of peptides into electrostatic polyelectrolyte complexes of polycations with proteins, 2) Inclusion of peptides into Cu(+2)-induced polyelectrolyte complexes of polyanions with similarly (anionic) charged proteins.

Immunogenic Cu2+-induced biopolymer systems comprising a steroid hormone, protein antigen, and synthetic polyelectrolytes.

We have synthesized the 17beta-estradiol comprising polycomplexes by the Cu(2+)-mediated complex formation of polyanionic polyelectrolyte (PE), [polyacrylic acid (PAA), nontoxic copolymers (CP) of acrylic acid with N-isopropylacrylamide and N-vinylpyrolidine] with BSA-estradiol covalent conjugates at the relatively low concentrations of metal ions in neutral water. Cu(2+) ions in the composition of biopolymer systems act as "fasteners" between macromolecules of same (negatively) charged polyanionic PE with protein carrier, promoting the formation of relatively stable polycomplex particles in physiological conditions. These hapten- and protein comprising Biopolymer Systems possess simultaneously highly estradiol and BSA-specific immunogenicity without traditional adjuvants after a single intravenous immunization of mice.

Fluorescence study of Cu(2+)-induced interaction between albumin and anionic polyelectrolytes.

The Cu(2+)-induced complex formation of bovine serum albumin (BSA) with anionic polyelectrolytes (PEs) (polyacrylic acid (PAA), poly(N-isopropylacrylamide) [poly[NIPAAm]], and copolymers of N-isopropylacrylamide (NIPAAm) and acrylic acid) in aqueous solution was studied by a fluorescence technique and high-performance liquid chromatography analysis. The character of the interactions depends on the monomer composition (r = [COOH]/[NIPAAm]), [Cu(2+)]/[PE], and [BSA]/[PE] ratios and solution pH. Two types of ternary polycomplex (polymer + Cu(2+) + BSA) particles are formed depending on the monomer composition r of the copolymer.

A novel aldehyde dextran sulfonate matrix for affinity biosensors.

Aldehyde dextran sulfonate (ADS), a modified oligosaccharide polymer, was used to prepare a new matrix structure for affinity biosensors. The principal difference between the ADS matrix and similar structures developed previously results from presence of two active functional groups in the matrix, namely, aldehyde and sulfonate. These groups perform two different functions in the matrix.

Water-soluble covalent conjugates of bovine serum albumin with anionic poly(N-isopropyl-acrylamide) and their immunogenicity.

We have conjugated bovine serum albumin (BSA) to poly(N-isopropylacrylamide-co-acrylic acid) (poly(NIPAAm-AA)) by using water-soluble carbodiimide, and the effects of the bulk mass ratio of protein to polymer (r) on the formation of polymer-protein conjugates have been studied. HPLC, electrophoresis, viscosimetry and fluorescence spectroscopy suggest that the mode of covalent binding of BSA to poly(NIPAAm-AA) depends upon the weight concentration ratio (r) of BSA to poly(NIPAAm-AA). At r < or = 1, free poly(NIPAAm-AA) molecules coexist with conjugate, and when r reaches 1 the amount of free polymer is too small to be observed.

Immune response to progesterone immobilized on Cu2+-induced amphifilic polyelectrolyte-protein complex: antigen specificity and affinity of hybridoma clones.

Cu2+-mediated complex formation between copolymers of acrylic acid with N-isopropyl-acyrlamide (CP1) and negatively charged covalent conjugate of bovine serum albumin with progesterone (BSA.P) was studied in neutral water in the presence of Cu2+. It was shown that under conditions where CP and BSA.

[Characteristics of tissue reaction upon implantation of injected polyacrylamide gel containing silver and hydroxyapatite].

Albino rats were subcutaneously injected with polyacrylamide gel (PAAG) (1 ml) with 20% distilled water, water with silver ions, and 1 and 3% hydroxyapatite (HAP), and tissue reaction was evaluated over 5-90 days postinjection. PAAG containing HAP and silver ions can be used for contour plastic repair of soft tissues; its use is particularly recommended for repair or modification of facial contours with one's own tissue.

Immune response to 17beta-estradiol involved in polymer gels: antigen specificity and affinity of hybridoma clones.

The immunogenic properties of 17beta-estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant.

[The clinical x-ray aspects of osteoplasty after osteotomy of the maxillary complex].

Forty-six patients with combined deformations of the jaws were subjected to surgical treatment. Formalin-treated bone allotransplants together with wire splints were found to play the retention and fixing role for a year after surgery. During this period complete consolidation of osteotomied fragment of the maxillary complex takes place.

Cu(2+)-mediated complex formation between polyacrylic acid (PAA) and bovine serum albumin.

Cu(2+)-mediated complex formation between polyacrylic acid (PAA) and negatively charged bovine serum albumin (BSA) was studied in neutral water in the presence of Cu2+. Depending on the concentration of Cu2+, the reaction between PAA-Cu2+ complexes and BSA appeared to follow one of two possible paths. At low Cu2+ concentrations (nCu/nAA < 0.

Immune response to progesterone involved in Cu(2+)-mediated polyanion-protein complex--antigen specificity and affinity of hybridoma clones.

The immunogenic properties of water soluble (PAA-Cu(2+)-BSA) and colloidal (PAA-Cu(2+)-BSA.P) polycomplexes were investigated, and the specificity of antibodies produced was analyzed. Polycomplexes containing progesterone appeared to possess a high steroid-specific immunogenic activity.

Immune response to 17 beta-estradiol in polyelectrolyte complex: antigen specificity and affinity of hybridoma clones.

Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R2, R16)], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water. Weakly water-soluble (colloidal) complex was formed upon addition of BSA.

[Possibility of using interferon in the treatment of alcoholism].

As many as 20 alcoholics were examined for the therapeutic efficacy of interferon. The control group was made up of 20 alcoholics who were treated by teturam. The clinical, neuroendocrine and immune data demonstrate the high efficacy of the use of interferon in alcoholics.

[Morphofunctional parallels in the dynamics of the take of an experimental free skin autograft].

Free skin autotransplantation may be adequately simulated in albino chinchilla rabbits. Gradual elevation of tc PO2 in the transplanted graft is considered as a functional indicator of the time course of autodermotransplant taking in. Morphologic study has shown dystrophic changes in the graft surface layers in the early periods of healing during scab formation, because of inadequate nutrition of the flap.

[Polymer-metal complexes of the Mycobacterium protein antigen prevent the dissemination of postvaccinal infection in the T-deficiency state].

Primary obtained triple highly immunogenetic complex consists of nonimmunogenic protein antigen, isolated from the BCG, nontoxic polyelectrolyte and bivalent cuprum ions. Immunization with this complex provides high immunoprotection in the experimented B mice (T-deficit mice) from lethal dissemination BCG. Inoculation of single components of the complex has no protection.

[The effect of structural-chemical characteristics of water-soluble polyelectrolyte complexes of ovalbumin on their immunological properties].

Immunogenicity of soluble protein antigens in the complexes with synthetic polyions may be regarded as depending both on the nature of polymer carrier and the structure of the protein-polyelectrolyte complex. The immunogenicity of stable soluble complexes of ovalbumin (OA) with polycation - quaternized poly-4-vinylpyridine (C-1) and copolymer of acrylic acid and 2-methyl-5-vinylpyridine (C-2) have been evaluated. Immunization of mice by C-1 have induced a vigorous formation of the anti-OA IgG antibodies and IgE homocytotropic antibodies, while immunogenicity of OA in C-2 was comparable with that of OA alone.

Polymer-metal complexes of protein antigens--new highly effective immunogens.

The mechanism of interaction of the copolymers N-vinylpyrrolidone-acrylic acid and N-vinylpyrrolidone-maleic anhydride with bovine serum albumin, influenza virus total surface antigen (haemagglutinin and neuraminidase), and the BCG protein fraction in the presence of divalent copper ions was investigated. Novel water-soluble triple polymer-metal complexes of the above protein antigens were formed. These complexes showed high immunogenicity and conferred high levels of immunological protection.