Publications by authors named "Mustafa O Baltaci"
Article Synopsis
- The study focuses on using waste frying oil as a carbon source to produce cost-effective rhamnolipids, overcoming common production barriers.
- Optimal conditions for rhamnolipid production were identified, resulting in a yield of 2.97 g/l at specific temperature, pH, and incubation time.
- The research indicates that using Halopseudomonas sabulinigri OZK5 for biosurfactant production is a promising biotechnological approach due to its eco-friendliness and efficiency.
Article Synopsis
- The study introduces a sustainable method for creating copper nanoparticles (Cu NPs) using enzymes from a thermophilic bacterium, offering a greener alternative to traditional synthesis methods.
- The production process utilized optimized conditions involving lignin peroxidase enzyme derived from peanut waste, emphasizing waste valorization and environmental benefits.
- Characterization of the Cu NPs confirmed their stability and shape, and they demonstrated significant antibacterial activity against various pathogens, particularly showing effectiveness against Streptococcus pyogenes, which suggests potential uses in medicine and other fields.
Article Synopsis
- Polyhydroxyalkanoates (PHAs) are being explored as eco-friendly alternatives to traditional plastics due to their biodegradability and biocompatibility, but cost-effective production remains a challenge.
- The study optimized production conditions using waste substrates, identifying Pseudomonas neustonica NGB15 as the most efficient bacteria under specific conditions (30 g/L banana peel powder, 25°C, pH 8, 4-day incubation).
- Results showed successful production of poly-β-hydroxybutyrate (3.34 g/L) highlighted by significant FTIR peaks, indicating the positive potential for low-cost fermentation in PHA bioproduction.
Article Synopsis
- Meat provides an ideal environment for foodborne pathogens like Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7, which can lead to serious health issues.
- A study surveyed 100 minced beef samples from different butchers in Erzurum province and used Real-Time PCR for pathogen detection, finding Listeria in 45 samples, E. coli in 30, and Salmonella in 29.
- The Real-Time PCR method showed higher efficiency and reliability compared to traditional techniques, allowing for quicker, more cost-effective, and less labor-intensive detection of these harmful bacteria.
Anoxybacillus: an overview of a versatile genus with recent biotechnological applications.
World J Microbiol Biotechnol
March 2023
Article Synopsis
- The Bacillaceae family, especially the genus Anoxybacillus, is gaining attention for its potential in biotechnological processes due to its ability to produce thermostable enzymes and spore-forming capabilities.
- Anoxybacillus, a relatively new genus since 2000, shows promise in applications like waste management and bioremediation, helping to break down toxic substances in various industrial processes.
- Recent research highlights the diverse Anoxybacillus strains from different environments and their various biotechnological applications, including enzyme production and the creation of bioactive exopolysaccharides with antimicrobial and anticancer properties.
Millions of tons of agricultural waste are produced globally every year. A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source.
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- Three-phase partitioning (TPP) is an effective method for purifying laccases, achieving a 4.9-fold purification and 110% recovery from a highly active A2 strain isolated from Agri-Diyadin hot spring.
- The A2 isolate was identified as Enterococcus faecium, showing impressive laccase characteristics, including a molecular mass of 50.11 kDa, optimal activity at pH 6.0 and 80 °C, and outstanding stability across a variety of environments.
- The enzyme demonstrated remarkable thermostability, maintained activity in harsh conditions with metal ions, surfactants, and organic solvents, and exhibited precise kinetic values when tested with the ABTS substrate.
Article Synopsis
- The study focuses on extracting chitin from shrimp shell powder using a specific bacteria, Paenibacillus jamilae, and making chitosan from it.
- The bacteria did not have chitinase but showed strong protease activity, with optimal conditions for deproteinization being pH 7.0, a shell concentration of 60 g/L, and an incubation period of 4 days.
- The resulting chitosan had a higher deacetylation degree and better antimicrobial and antioxidant properties compared to commercial chitosan, marking a significant finding in biological chitin extraction.
Article Synopsis
- The study examined how different temperatures of NaOCl irrigation affect postoperative pain and bacteria levels in root canal treatments.
- Results showed that all temperature groups significantly reduced bacterial count, but the group with cold NaOCl (2˚C) experienced less postoperative pain compared to the warmer NaOCl (45˚C).
- Overall, while antibacterial effectiveness was similar across temperatures, cold NaOCl was found to be more beneficial for minimizing pain after treatment.
A novel Gram-stain-positive, rod-shaped, endospore-forming, motile, aerobic bacterium, designated as P2, was isolated from a hot spring water sample collected from Ilica-Erzurum, Turkey. Phylogenetic analyses based on 16S rRNA gene sequence comparisons affiliated strain P2 with the genus , and the strain showed the highest sequence identity to NBRC 15712 (96.7 %).
View Article and Find Full Text PDFsp. nov., a thermophilic bacterium isolated from a hot spring in Turkey.
Int J Syst Evol Microbiol
June 2020
Article Synopsis
- Strain P1, a Gram-positive, endospore-forming bacterium, was isolated from Pasinler Hot Spring and characterized to determine its taxonomic classification.
- It shares high genetic similarity with known strains R-6488 (97.6%) and MO-04 (97.2%), and forms a unique group in phylogenetic analyses based on 16S rRNA gene sequences.
- Strain P1 thrives at 55°C and 2% NaCl, contains specific cell-wall components, and is proposed as a new species within the genus, named sp. nov., with type strain P1.
Article Synopsis
- An extracellular thermostable alkaline serine protease enzyme was isolated from Aeribacillus pallidus C10, achieving a purification enhancement of 4.85 to 17.32 times with yields between 19.56% and 26.9%.
- The enzyme has a molecular weight of approximately 38.35 kDa and shows peak activity at pH 9 and 60°C, remaining stable within a pH range of 7.0-10.0 and retaining over 80% activity between 20-80°C.
- It also maintains significant activity in the presence of organic solvents and commercial detergents, with activity boosted by the presence of 5% SDS; kinetic parameters include K and V values of 0
Article Synopsis
- The study focused on purifying an extracellular thermostable alkaline protease from the A10 strain, achieving a 1.38-fold increase in purity with 9.44% efficiency using ammonium sulfate precipitation and DE52 anion exchange chromatography.
- The enzyme has a molecular weight of approximately 40.55 kDa, with optimal activity at pH 9.0 and a temperature of 70°C, showing stability in a pH range of 7.5-10.5 and maintaining over 90% activity after 1 hour at 60-70°C.
- Certain metal ions, like Mg(2+), Mn(2+), and K(+), enhanced the enzyme's activity