Hydroxyapatite-decorated ZrO for α-amylase immobilization: Toward the enhancement of enzyme stability and reusability.

Herein, the immobilization of α-amylase onto hydroxyapatite (HA) and hydroxyapatite-decorated ZrO (10%wt) (HA-ZrO) nanocomposite were investigated. The immobilization yield was 69.7% and 84% respectively.

Dual immobilization of α-amylase and horseradish peroxidase via electrospinning: A proof of concept study.

In this work, we propose a facile technique to dually-immobilize α-amylase and horseradish peroxidase (HRP) as two different enzyme models via entrapment within two distinct polymeric electrospun fibers by simple mixing steps and compare their properties with both individually immobilized forms and with the free counterparts. The immobilization was verified using Fourier transform infrared spectroscopy (FTIR) and Field emission scanning electron microscope (FESEM). The immobilization efficiencies for the dual-immobilized HRP and α-amylase were 89% and 85%, respectively.

Immobilisation of α-amylase on activated amidrazone acrylic fabric: a new approach for the enhancement of enzyme stability and reusability.

In this study, amidrazone acrylic fabric was applied as an immobilising support for α-amylase. The immobilised α-amylase was characterised by Fourier transform infrared spectroscopy and scanning electron microscopy. Furthermore, the optimum conditions for immobilisation efficiency, immobilisation time, reusability, kinetic parameters and pH, for the immobilisation process were examined.

Amidrazone modified acrylic fabric activated with cyanuric chloride: A novel and efficient support for horseradish peroxidase immobilization and phenol removal.

In this study, hydrazine treated acrylic fabrics (polyacrylonitrile, PAN) activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The immobilization of HRP onto the modified supporting material was achieved after being end-over-end incubated for 12 h. Field emission scanning electron microscopy and Fourier-transform infrared spectroscopy techniques were used to confirm the successful immobilization.

α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride.

Enzyme immobilization is one of the most important techniques for industrial applications. It makes the immobilized enzyme more stable and advantageous than the free form in different aspects. α-Amylase was immobilized on 4% cyanuric chloride-activated amidoximated acrylic fabric at pH 7.

Multifunctional magnetic-gold nanoparticles for efficient combined targeted drug delivery and interstitial photothermal therapy.

Abundant efforts have recently been made to design potent theranostic nanoparticles, which combine diagnostic and therapeutic agents, for the effective treatment of cancer. In this study, we developed multifunctional magnetic gold nanoparticles (MGNPs) that are able to (i) selectively deliver the drug to the tumor site in a controlled-release manner, either passively or by using magnetic targeting; (ii) induce photothermal therapy by producing heat by near-infrared (NIR) laser absorption; and (iii) serve as contrast agents for magnetic resonance imaging (MRI) (imaging-guided therapy). The prepared MGNPs were characterized by different physical techniques.

Immobilization of horseradish peroxidase on PMMA nanofibers incorporated with nanodiamond.

In the present study, nanodiamond (ND) was blended with polymethyl methacrylate (PMMA) and then electrospun into nanofibers (nfPMMA-ND) for the immobilization of horseradish peroxidase (HRP). The maximum immobilization efficiency of HRP (96%) was detected at 10% ND and pH 7.0.

Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor.

The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized α-amylases with regard to pH, thermal stability, and reusability were studied.

Encapsulation of 5-Flurouracil into PLGA Nanofibers and Enhanced Anticancer Effect in Combination with Ajwa-Dates-Extract (.).

Side effects connected with chemotherapeutic agents used in cancer treatment has led to alternative modalities of combinatorial therapies in an attempt to reduce the drug dosage and associated risks. In the current study we evaluated the potential use of Ajwa Dates Extract (ADE), reported to have anti-cancer effects, as an adjuvant therapy in combination with 5-flurouracil (5FU) against the human-breast-adenocarcinoma cell line (MFC-7) in vitro. The effects of ADE alone and in combination with 5-FU were evaluated in terms of cell viability and cytotoxicity.

Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery.

Delivery of drugs with controlled temporal profiles is essential for wound treatment and regenerative medicine applications. For example, bacterial infection is a key challenge in the treatment of chronic and deep wounds. Current treatment strategies are based on systemic administration of high doses of antibiotics, which result in side effects and drug resistance.

Bioprinting technologies for disease modeling.

There is a great need for the development of biomimetic human tissue models that allow elucidation of the pathophysiological conditions involved in disease initiation and progression. Conventional two-dimensional (2D) in vitro assays and animal models have been unable to fully recapitulate the critical characteristics of human physiology. Alternatively, three-dimensional (3D) tissue models are often developed in a low-throughput manner and lack crucial native-like architecture.

Nanofibrous Silver-Coated Polymeric Scaffolds with Tunable Electrical Properties.

Electrospun micro- and nanofibrous poly(glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) substrates have been extensively used as scaffolds for engineered tissues due to their desirable mechanical properties and their tunable degradability. In this study, we fabricated micro/nanofibrous scaffolds from a PGS-PCL composite using a standard electrospinning approach and then coated them with silver (Ag) using a custom radio frequency (RF) sputtering method. The Ag coating formed an electrically conductive layer around the fibers and decreased the pore size.

Hydrogels 2.0: improved properties with nanomaterial composites for biomedical applications.

The incorporation of nanomaterials in hydrogels (hydrated networks of crosslinked polymers) has emerged as a useful method for generating biomaterials with tailored functionality. With the available engineering approaches it is becoming much easier to fabricate nanocomposite hydrogels that display improved performance across an array of electrical, mechanical, and biological properties. In this review, we discuss the fundamental aspects of these materials as well as recent developments that have enabled their application.