Publications by authors named "Murong Du"

Single-cell metabolic analysis has not yet achieved the coverage of bulk analysis due to the diversity of cellular metabolites and the ionization competition among species. Direct ionization methods without separation lead to the masking of low-intensity species. By designing a capillary column emitter and introducing reverse-phase chromatography principles, we achieved the microseparation of lipophilic and hydrophilic metabolites and lowered the limit of detection of hydrophilic metabolites to the level of a single oocyte.

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Article Synopsis
  • A new open-source Python library called SCMeTA has been developed to improve single-cell metabolomics (SCM) research by standardizing data processing and enhancing communication between components.
  • The library includes modular features to cater to various SCM study requirements and has been validated against multiple SCM experiment datasets, showing improvements in accuracy, metabolic extraction, and processing speed.
  • SCMeTA is freely available online, promoting its use in advancing biological studies and broadening the application of SCM analysis.
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Chemoresistance to triple-negative breast cancer (TNBC) is a critical issue in clinical practice. Lipid metabolism takes a unique role in breast cancer cells; especially, unsaturated lipids involving cell membrane fluidity and peroxidation are highly remarked. At present, for the lack of a high-resolution molecular recognition platform at the single-cell level, it is still hard to systematically study chemoresistance heterogeneity based on lipid unsaturation proportion.

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Lymphocytes play crucial roles in the human immune system; however, detailed metabolite characteristics need to be further investigated. Herein, we propose a lymphocyte classification method based on metabolite profiling at the single-cell level. The percentages of different lymphocyte types were calculated with a low margin of error, confirming that the metabolites could serve as a basis for lymphocyte classification.

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