Development of marker-free insect resistant transgenic okra ( L. Moench) expressing the gene and identification of vector backbone-free events.

-mediated co-transformation method was used to generate marker-free insect resistant transgenic okra plants expressing the gene. The gene was borne on the T-DNA of one plasmid while II and (GUS) marker genes were present on the T-DNA of a second plasmid. Putative transgenic plants were screened by histochemical GUS assay for expression of -glucuronidase and 32 transgenic events were positive for GUS in which 21 transgenic events were positive in ELISA for the presence of Cry1Ac protein.