Expression of glycodelin and cyclooxygenase-2 in human endometrial tissue following three-dimensional culture.

Problem: Our previous study showed that in vitro culture of human endometrial tissue in a three-dimensional (3D) fibrin matrix could mimic the early stages of endometriosis with invasion, gland and stroma formation and sprouting of new vessels. The objective of the present study was to evaluate the expression of glycodelin (Gd) and cyclooxygenase-2 (COX-2), two angiogenic factors, to further validate the 3D culture model of endometriosis.

Method Of Study: Human endometrial fragments were obtained from endometrial biopsies and placed in a 3D fibrin matrix culture.

Complete failed fertilization after intracytoplasmic sperm injection--analysis of 10 years' data.

Objective: To investigate incidence and causes of complete failed fertilization after intracytoplasmic sperm injection (ICSI) in a tertiary care facility.

Methods: A total of 1,779 cycles between February 1994 and December 2003 were analyzed. Study parameters were female age, infertility diagnosis, ovarian stimulation protocol, estradiol level on day of hCG administration, number of follicles, number of oocytes retrieved, number of oocytes injected, and semen parameters.