Can generative AI replace immunofluorescent staining processes? A comparison study of synthetically generated cellpainting images from brightfield.

Cell imaging assays utilising fluorescence stains are essential for observing sub-cellular organelles and their responses to perturbations. Immunofluorescent staining process is routinely in labs, however the recent innovations in generative AI is challenging the idea of wet lab immunofluorescence (IF) staining. This is especially true when the availability and cost of specific fluorescence dyes is a problem to some labs.

Continuous-wave driving elucidates the desynchronization dynamics of ultrashort dissipative Raman solitons generated in dispersive Kerr resonators.

Phase-coherent pulsed driving of passive optical fiber resonators enables the generation of ultrashort dissipative Raman solitons with durations well below 100 fs. The existence and characteristics of such solitons critically depend on the desynchronization between the pulsed driving source and the resonator round trip time, yet the full mechanism through which these dependencies arise remains unclear. Here, we numerically demonstrate that Raman solitons can exist even under conditions of continuous-wave (CW) driving, and by numerically examining the existence and characteristics of Raman solitons under such conditions, we elucidate the role of desynchronization in pulse-driven systems.

Dietary fibre supplementation enhances radiotherapy tumour control and alleviates intestinal radiation toxicity.

Background: Non-toxic approaches to enhance radiotherapy outcomes are beneficial, particularly in ageing populations. Based on preclinical findings showing that high-fibre diets sensitised bladder tumours to irradiation by modifying the gut microbiota, along with clinical evidence of prebiotics enhancing anti-cancer immunity, we hypothesised that dietary fibre and its gut microbiota modification can radiosensitise tumours via secretion of metabolites and/or immunomodulation. We investigated the efficacy of high-fibre diets combined with irradiation in immunoproficient C57BL/6 mice bearing bladder cancer flank allografts.

Immunohistochemistry for Diagnosing Melanoma in Older Adults.

Importance: Pathologic assessment to diagnose skin biopsies, especially for cutaneous melanoma, can be challenging, and immunohistochemistry (IHC) staining has the potential to aid decision-making. Currently, the temporal trends regarding the use of IHC for the examination of skin biopsies on a national level have not been described.

Objective: To illustrate trends in the use of IHC for the examination of skin biopsies in melanoma diagnoses.

A treatable inborn error of metabolism presenting in the sixth decade.

Phenylketonuria (PKU) is an inborn error of amino acid metabolism. If untreated, PKU can result in global developmental delay, learning difficulties or seizures. For that reason, PKU is included in the UK neonatal screening programme.

Nonlinear topological symmetry protection in a dissipative system.

We investigate experimentally and theoretically a system ruled by an intricate interplay between topology, nonlinearity, and spontaneous symmetry breaking. The experiment is based on a two-mode coherently-driven optical resonator where photons interact through the Kerr nonlinearity. In presence of a phase defect, the modal structure acquires a synthetic Möbius topology enabling the realization of spontaneous symmetry breaking in inherently bias-free conditions without fine tuning of parameters.

Kerr microresonator dual-comb source with adjustable line-spacing.

Optical microresonators offer a highly-attractive new platform for the generation of optical frequency combs. Recently, several groups have been able to demonstrate the generation of dual-frequency combs in a single microresonator driven by two optical pumps. This opens the possibility for microresonator-based dual-comb systems suitable for measurement applications such as spectroscopy, ranging and imaging.

Pharmacokinetic Model-Based Control across the Blood-Brain Barrier for Circadian Entrainment.

The ability to shift circadian phase in vivo has the potential to offer substantial health benefits. However, the blood-brain barrier prevents the absorption of the majority of large and many small molecules, posing a challenge to neurological pharmaceutical development. Motivated by the presence of the circadian molecule KL001, which is capable of causing phase shifts in a circadian oscillator, we investigated the pharmacokinetics of different neurological pharmaceuticals on the dynamics of circadian phase.

Temporal characteristics of stationary switching waves in a normal dispersion pulsed-pump fiber cavity.

Kerr cavities driven in the normal dispersion regime are known to host switching waves. These consist of a traveling wavefront that connects separate regions associated with high- and low-intensity steady states of the cavity. In this Letter, we drive a 230-m custom built fiber ring cavity with strong normal dispersion using nanosecond pulses, allowing us to directly resolve the fine structure of individual switching waves, including resonant oscillations occurring over periods of the order of ∼10 ps.

Random number generation using spontaneous symmetry breaking in a Kerr resonator.

We demonstrate an all-optical random number generator based on spontaneous symmetry breaking in a coherently driven Kerr resonator. Random bit sequences are generated by repeatedly tuning a control parameter across a symmetry-breaking bifurcation that enacts random selection between two possible steady-states of the system. Experiments are performed in a fiber ring resonator, where the two symmetry-broken steady-states are associated with orthogonal polarization modes.

Inter-mode soliton linear-wave scattering in a Kerr microresonator.

Soliton microresonator frequency combs (microcombs) have recently emerged as an attractive new type of optical comb source with a wide range applications proposed and demonstrated. To extend the optical bandwidth of these microresonator sources, several previous studies have proposed and studied the injection of an additional optical probe wave into the resonator. In this case, nonlinear scattering between the injected probe and the original soliton enables the formation of new comb frequencies through a phase-matched cascade of four-wave mixing processes.

Impact of early waves of the COVID-19 pandemic on family medicine residency training: Analysis of survey data.

Objective: To identify how graduating and incoming family medicine residents (FMR) experienced changes to their education during the early waves of the COVID-19 pandemic.

Design: The Family Medicine Longitudinal Survey was modified with questions related to the impact of COVID-19 on FMR and their training. Short-answer responses underwent thematic analysis.

Wideband multimode optical parametric oscillation in a Kerr microresonator.

Parametric oscillation in Kerr microresonators provides an attractive pathway for the generation of new optical frequencies in a low-power, small-footprint device. The frequency shift of the newly generated parametric sidebands is set by the phasematching of the underlying four-wave-mixing process, with the generation of large frequency shift sidebands typically placing exacting requirements on a resonator's dispersion profile. In practice, this limits the range of viable pump wavelengths, and ultimately the range of output frequencies.

Feeling known and informed: Serial qualitative interviews evaluating a consultation support intervention for patients with high-grade glioma.

Objective: Interventions to support patients' engagement in shared decision making (SDM) are lacking within high-grade glioma (HGG) healthcare. Consultation Planning, Recording and Summarising (CPRS) has shown evidence of increasing patient decision self-efficacy, reducing uncertainty, and regret of decisions. This is the first study of CPRS within a HGG population and delivered over serial medical consultations.

A scoping review of COVID-19 online mis/disinformation in Black communities.

Background: Mis/disinformation has reached an epidemic level with the COVID-19 virus and can be largely attributed to the growing digitalization of information and its rapid transmission via social media. Approximately 96% of Canadians and 80% of Americans report encountering COVID-19 dis/misinformation on at least one social media site/app. COVID-19 dis/misinformation promotes scepticism and a lack of confidence in COVID-19 interventions.

Barriers and facilitators to physical activity participation for child, adolescent, and young adult cancer survivors: a systematic review.

Purpose: The aim of this systematic review is to explore and describe the barriers and facilitators to physical activity (PA) participation for child, adolescent, and young adult cancer survivors.

Methods: MEDLINE, Embase, AMED, CINAHL, Cochrane, and Web of Science were searched for manuscripts published between January 2000 and February 2021. To be included in this review, studies had to report qualitative or quantitative data on barriers and facilitators to PA participation in child (4 to < 10 years), adolescent (10 to 19 years), and young adult (> 19 to < 40 years) cancer survivors.

Breathing dynamics of symmetry-broken temporal cavity solitons in Kerr ring resonators.

We investigate theoretically and experimentally the instabilities of symmetry-broken, vectorial, bright cavity solitons (CSs) of two-mode nonlinear passive Kerr resonators. Through comprehensive theoretical analyses of coupled Lugiato-Lefever equations, we identify two different breathing regimes where the two components of the vectorial CSs breathe respectively in-phase and out-of-phase. Moreover, we find that deep out-of-phase breathing can lead to intermittent self-switching of the two components, spontaneously transforming a soliton into its mirror-symmetric state.

The LeVe CPAP System for Oxygen-Efficient CPAP Respiratory Support: Development and Pilot Evaluation.

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has placed a significant demand on healthcare providers (HCPs) to provide respiratory support for patients with moderate to severe symptoms. Continuous Positive Airway Pressure (CPAP) non-invasive ventilation can help patients with moderate symptoms to avoid the need for invasive ventilation in intensive care. However, existing CPAP systems can be complex (and thus expensive) or require high levels of oxygen, limiting their use in resource-stretched environments.

Dual-microcomb generation in a synchronously driven waveguide ring resonator.

Microcombs-optical frequency combs generated in coherently driven nonlinear microresonators-have attracted significant attention over the last decade. The ability to generate two such combs in a single resonator device has, in particular, enabled a host of applications from spectroscopy to imaging. Concurrently, novel comb generation techniques such as synchronous pulsed driving have been developed to enhance the efficiency and flexibility of microcomb generation.