Iodouracil-mediated photocrosslinking of DNA to EcoRII restriction endonuclease in catalytic conditions.

We used a XeCl excimer laser with 50 ns pulses, a frequency of 0.3 Hz and a wavelength of 308 nm in appropriate conditions for the photocrosslinking of EcoRII restriction endonuclease to a 14-mer DNA duplex, containing a 5-iodo-2'-deoxyuridine residue (IdU). IdU replaced the thymidine residue within the EcoRII recognition sequence 5'-CCT/AGG.