Valorization of shrimp processing waste-derived chitosan into anti-inflammatory chitosan-oligosaccharides (CHOS).

Bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using a Bacillus chitosanase, BsCsn46A, was investigated, including food-grade approaches. After 48 h of enzymatic reaction, most of the final products were dimers and trimers. None of the CHOS products showed toxicity to human fibroblasts.

Anti-inflammatory activity of soluble chito-oligosaccharides (CHOS) on VitD3-induced human THP-1 monocytes.

Chito-oligosaccharides (CHOS) are oligomers of D-glucosamine and N-acetyl-glucosamine. Anti-inflammatory activities of a wide variety of CHOS mixtures have previously been reported, mainly based on studies with mouse models and murine macrophages. Since the mouse and human immune systems are quite different, gaining insight into the activity of CHOS on human cell lines, using well-characterized CHOS mixtures, is of considerable interest.

Sesamol induces mitochondrial apoptosis pathway in HCT116 human colon cancer cells via pro-oxidant effect.

Aims: Sesamol lignan is a phenolic compound found in sesame seeds. We investigated the effect of different concentrations of sesamol on oxidative stress in colorectal carcinoma cells (HCT116).

Main Methods: Antioxidation in vitro was determined from elimination of the DPPH radical, ferric reducing-antioxidant power (FRAP), O2(-), and peroxyl radical scavenging activity.