Three-year clinical effectiveness of a two-step self-etch adhesive in cervical lesions.

A 3-yr randomized, controlled prospective study evaluated the clinical effectiveness of a mild two-step self-etch adhesive, Clearfil SE, in Class-V non-carious lesions. The hypothesis tested was that prior selective etching of enamel with phosphoric acid does not affect the 3-yr clinical performance of this adhesive. A total of 100 lesions in 29 patients were randomly restored in one or two pairs, according to two experimental protocols: (i) application of Clearfil SE according to the instructions of the manufacturer (C-SE non-etch); and (ii) similar application of Clearfil SE with prior etching of enamel cavity margins with phosphoric acid (C-SE etch).

Hydrolytic stability of self-etch adhesives bonded to dentin.

Functional monomers chemically interact with hydroxyapatite that remains within submicron hybrid layers produced by mild self-etch adhesives. The functional monomer 10-MDP interacts most intensively with hydroxyapatite, and its calcium salt appeared most hydrolytically stable, as compared with 4-MET and phenyl-P. We investigated the hypothesis that additional chemical interaction of self-etch adhesives improves bond stability.

Extension of a one-step self-etch adhesive into a multi-step adhesive.

Unlabelled: One-step self-etch adhesives are undoubtedly the most user-friendly adhesives, but have been associated with lower bonding effectiveness as compared to two-step and three-step adhesives. Conversion of a one-step self-etch system into a two-step self-etch adhesive by adding a bonding step, or into a three-step etch&rinse adhesive by adding a beforehand etching step and a bonding step might be tempting in order to improve bond strength.

Objectives: The objective of this study was to investigate whether adding application steps influences the bond strength of a one-step self-etch adhesive.

Correlating the alpha rhythm to BOLD using simultaneous EEG/fMRI: inter-subject variability.

Simultaneous recording of electroencephalogram/functional magnetic resonance images (EEG/fMRI) was applied to identify blood oxygenation level-dependent (BOLD) changes associated with spontaneous variations of the alpha rhythm, which is considered the hallmark of the brain resting state. The analysis was focused on inter-subject variability associated with the resting state. Data from 7 normal subjects are presented.

How do brain tumors alter functional connectivity? A magnetoencephalography study.

Objective: This study was undertaken to test the hypothesis that brain tumors interfere with normal brain function by disrupting functional connectivity of brain networks.

Methods: Functional connectivity was assessed by computing the synchronization likelihood in a broad band (0.5-60Hz) or in the gamma band (30-60Hz) between all pairwise combinations of magnetoencephalography signals.

Micro-tensile bond strength of adhesives bonded to Class-I cavity-bottom dentin after thermo-cycling.

Unlabelled: A widely used artificial aging methodology is thermo-cycling. The ISO TR 11450 standard (1994) recommends 500 cycles in water between 5 and 55 degrees C. Recent literature revealed that more cycles are needed to mimic long-term bonding effectiveness.

Effect of evaporation of primer components on ultimate tensile strengths of primer-adhesive mixture.

Objective: The purpose of the present study was to evaluate the effect of evaporation of primer components on the ultimate tensile strength (UTS) of primer-adhesive mixtures.

Methods: The UTSs of 1:3 by weight primer-adhesive mixtures and of the pure adhesives (controls) were measured after 0-, 2-, 5-min and 24-h storage in a 37 degrees C oven for the three-step etch&rinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Clearfil SE (Kuraray).

Results: Evaporation of primer components increased with time for both adhesives investigated.

Comparison of liquid based cytology and histology for the evaluation of HER-2 status using immunostaining and CISH in breast carcinoma.

Background: HER-2 amplification is an important prognostic biomarker and treatment determinant in breast carcinoma.

Aims: To correlate immunocytochemical (ICC) expression of HER-2 and gene amplification determined by chromogenic in situ hybridisation (CISH) using liquid based cytology (LBC) with immunohistochemistry (IHC) and CISH using histological samples of the same breast carcinomas.

Methods: Frozen sections and cytobrushings of 103 breast carcinomas were analysed.

In vivo degradation of resin-dentin bonds produced by a self-etch and an etch-and-rinse adhesive.

Resin-dentin bonds are known to degrade in the relatively aggressive oral environment. In order to obtain greater insight into the interfacial degradation process, we examined, by using transmission electron microscopy (TEM), the interfacial ultrastructure of two adhesives bonded to dentin after 1 yr in vivo. Class V cavities were prepared on the buccal surfaces of 14 intact teeth of two monkeys and then restored by using either the two-step self-etch adhesive, Unifil Bond, or the two-step etch-and-rinse adhesive, Single Bond, in combination with the restorative microhybrid composite, Z250.

The spatiotemporal MEG covariance matrix modeled as a sum of Kronecker products.

The single Kronecker product (KP) model for the spatiotemporal covariance of MEG residuals is extended to a sum of Kronecker products. This sum of KP is estimated such that it approximates the spatiotemporal sample covariance best in matrix norm. Contrary to the single KP, this extension allows for describing multiple, independent phenomena in the ongoing background activity.

Clinical effectiveness of contemporary adhesives: a systematic review of current clinical trials.

Objectives: The purpose of this paper was to review current literature on the clinical effectiveness of contemporary adhesives when used to restore cervical non-carious class-V lesions. Restoration retention in function of time was recorded in order to find out if adhesives with a simplified application procedure are as clinically effective as conventional three-step adhesives.

Data Sources: Literature published from January 1998 up to May 2004 was reviewed for university-centred clinical trials that tested the clinical effectiveness of adhesives in non-carious class-V lesions.

Bond strength of a mild self-etch adhesive with and without prior acid-etching.

Unlabelled: The self-etch approach provides dentists with a generation of user-friendly and less technique-sensitive adhesives. Nevertheless, some concern has been raised regarding their bonding effectiveness to enamel, in particular when so-called 'mild' self-etch adhesives are employed.

Objectives: The purpose of this study was to test the hypothesis that the two-step self-etch adhesive Clearfil SE Bond (C-SE; Kuraray, Osaka, Japan) bonds equally effective to enamel/dentin either with or without prior etching with phosphoric acid.

Age distribution of MEG spontaneous theta activity in healthy subjects.

This study investigates the possible relevance of distribution and age variation of spontaneous theta activity (4-8 Hz) in normal subjects using magnetoencephalography (MEG) recordings. Spontaneous theta was recorded with a 151-channel MEG in healthy subjects; moreover, in a group of 10 subjects, simultaneous MEG-EEG was recorded in order to compare the two methods. Theta was divided in two sub-bands: T(A) (4-6 Hz) and T(B) (6-8 Hz).

Technique-sensitivity of contemporary adhesives.

Besides micro-mechanical interlocking through hybrid-layer formation, self-etch adhesives may benefit from additional chemical interaction between the functional monomer and residual hydroxyapatite. One-step adhesives are commonly associated with lower bonding effectiveness, which must be attributed in part to the dissolution of hydrophilic and hydrophobic monomers in a relatively highly concentrated solvent. In this 'difficult' mixture, also water is essential as ionization medium to enable self-etching activity.

Effect of fracture strength of primer-adhesive mixture on bonding effectiveness.

Objective: The purpose of this study was to measure the micro-tensile bond strength (microTBS) of two adhesives to dentin and to correlate this microTBS with the micro-tensile fracture strength (microTFS) of primer-adhesive mixtures of the two adhesives.

Methods: The three-step etch&rinse adhesive OptiBond FL (Kerr) and the two-step self-etch adhesive Clearfil SE Bond (Kuraray) were used to bond Filtek Z100 (3M ESPE) to twenty mid-coronal dentin surfaces. The microTBS was determined at 1 and 24 h after light-curing.

Differences in MEG/EEG epileptic spike yields explained by regional differences in signal-to-noise ratios.

Controversy remains regarding the preferred modality, magnetoencephalography (MEG) or EEG, for the presurgical evaluation of patients with epilepsy. In general, it appears that the spike yields for MEG and EEG are similar in patients with temporal lobe epilepsy, and that for neocortical epilepsy the MEG spike yields may be larger than for EEG. In general, MEG/EEG spike yields depend on factors such as (1) the number of sensors, (2) the source depth and orientation, (3) the background activity, and (4) the smearing of the potential fields due to variations in skull resistivity in EEG.

A randomized controlled study evaluating the effectiveness of a two-step self-etch adhesive with and without selective phosphoric-acid etching of enamel.

Objectives: The purpose of this randomized controlled clinical trial was to test the hypothesis that a two-step self-etch approach is equally effective to restore cervical class-V lesions as a self-etch approach with beforehand selective etching of enamel using phosphoric acid.

Methods: Twenty-nine patients received two or four restorations randomly following two experimental protocols ('paired-tooth' study design): (1) A 'mild' self-etch adhesive (Clearfil SE, Kuraray) was applied following a self-etch approach on both enamel and dentin (C-SE non-etch); (2) Similar application of Clearfil SE, but including beforehand selective acid-etching of the enamel cavity margins with 40% phosphoric acid (C-SE etch). Clearfil AP-X (Kuraray) was used as restorative composite for all 100 restorations.

One-day bonding effectiveness of new self-etch adhesives to bur-cut enamel and dentin.

Self-etch adhesives try to solve difficulties commonly associated with the clinical application of etch-and-rinse adhesives. Their application procedure is considered less time-consuming and, more importantly, less technique-sensitive. The main objective of this study was to determine the bonding effectiveness to and the interaction with enamel/dentin of three contemporary one- and two-step self-etch adhesives by microtensile bond strength testing (microTBS), Fe-SEM and TEM when compared to a control two-step self-etch and a three-step etch-and-rinse adhesive.

Fatigue resistance of dentin/composite interfaces with an additional intermediate elastic layer.

According to the "elastic bonding" concept, a thick intermediate layer of flexible resin has been suggested to absorb part of the polymerization shrinkage stress and to absorb shocks during function. In this study, the effect of an additional intermediate layer of a low-viscosity resin on the microrotary fatigue resistance (microRFR) of a hybrid composite bonded to dentin was evaluated. The hypotheses tested were that an intermediate layer of a low-viscosity resin (i) increases the microRFR to dentin, but (ii) has no effect on the static bond strength.

What does an epileptiform spike look like in MEG? Comparison between coincident EEG and MEG spikes.

Recent investigations suggest that there are differences between the characteristics of EEG and MEG epileptiform spikes. The authors performed an objective characterization of the morphology of epileptiform spikes recorded simultaneously in both EEG and MEG to determine whether they present the same morphologic characteristics. Based on a stepwise approach, the authors performed a computer analysis of EEG and MEG of a set of coincident epileptiform transients selected by a senior clinical neurophysiologist in recordings of three patients with drug-resistant epilepsy.

Effect of cavity configuration and aging on the bonding effectiveness of six adhesives to dentin.

Objectives: The purpose of this study was to determine the effect polymerization contraction stress may have on bond durability.

Methods: Bonding effectiveness was assessed by micro-tensile bond strength testing (muTBS) and electron microscopy. The muTBS to flat dentin surfaces and in standardized cavities was determined (this after 1 day as well as 1 year water storage).

Monomer-solvent phase separation in one-step self-etch adhesives.

One-step adhesives bond less effectively to enamel/dentin than do their multi-step versions. To investigate whether this might be due to phase separation between adhesive ingredients, we characterized the interaction of 5 experimental and 3 commercial self-etch adhesives with dentin using transmission electron microscopy. All adhesives were examined for homogeneity by light microscopy.

A critical review of the durability of adhesion to tooth tissue: methods and results.

The immediate bonding effectiveness of contemporary adhesives is quite favorable, regardless of the approach used. In the long term, the bonding effectiveness of some adhesives drops dramatically, whereas the bond strengths of other adhesives are more stable. This review examines the fundamental processes that cause the adhesion of biomaterials to enamel and dentin to degrade with time.

Effect of conditioner on bond strength of glass-ionomer adhesive to dentin/enamel with and without smear layer interposition.

The effect of a polyalkenoic acid conditioner pretreatment on the bond strength of a glass-ionomer adhesive to tooth substrates with or without smear layer was evaluated. Smear-layer covered and smear-layer free dentin and enamel surfaces were prepared from 24 extracted human molars. Resin composite was bonded to the surfaces using FujiBond LC with or without a polyalkenoic acid conditioner and subjected to microtensile bond strength (microTBS) testing.

A maximum-likelihood estimator for trial-to-trial variations in noisy MEG/EEG data sets.

The standard procedure to determine the brain response from a multitrial evoked magnetoencephalography (MEG) or electroencephalography (EEG) data set is to average the individual trials of these data, time locked to the stimulus onset. When the brain responses vary from trial-to-trial this approach is false. In this paper, a maximum-likelihood estimator is derived for the case that the recorded data contain amplitude variations.