Comparative Genomic Analysis Reveals the Emergence of ST-231 and ST-395 Strains Associated with the High Transmissibility of Plasmids.

Conjugative transposons in Gram-negative bacteria have a significant role in the dissemination of antibiotic-resistance-conferring genes between bacteria. This study aims to genomically characterize plasmids and conjugative transposons carrying integrons in clinical isolates of genetic composition of conjugative transposons and phenotypic assessment of 50 multidrug-resistant isolates from a tertiary-care hospital (SQUH), Muscat, Oman, were investigated. Horizontal transferability was investigated by filter mating conjugation experiments.

Exploring Synergistic Combinations in Extended and Pan-Drug Resistant (XDR and PDR) Whole Genome Sequenced .

Unlabelled: The diminishing antimicrobial options for the treatment of XDR and PDR is an increasing concern. In this study, we assessed the in vitro synergy of the fosfomycin (FOS) with meropenem (MEM), amikacin (AK), tigecycline (TGC), and colistin (CL) in whole genome sequenced isolates. Non-replicate whole genome sequenced (illumina next-generation sequencing platform, Clevergene, India), (7 XDR, 1PDR) were subjected to in vitro synergy testing by checkerboard (CB) and time kill assay (TKA) after MIC determination, with glucose-6-phosphate being incorporated in all runs.