Effects of structural variation in electrospray systems on spray characteristics.

Electrospraying is a method of atomizing fluids using a high voltage potential difference. Since the atomization of electrostatic atomization does not directly charge the nozzle but only uses the potential difference, various equipment structures are possible. In the case of this study, 12 different equipment structures of nozzle, ring electrode, and substrate were configured, and data on atomization characteristics of electrospray, such as spray modes applied voltage, coating area, coating current, spray velocity, and factors used in industrial processes, were verified.

Experimental Investigation of Electrospraying Properties Based on Ring Electrode Modification.

Electrospraying uses a high-voltage potential difference to create fine droplets. This study conducts a comparative analysis of the spray pattern and droplet properties using ring electrode parameters. The spray pattern and droplet characteristics are analyzed based on the experimental parameters of the ring electrode.

Repurposing type III polyketide synthase as a malonyl-CoA biosensor for metabolic engineering in bacteria.

Malonyl-CoA is an important central metabolite for the production of diverse valuable chemicals including natural products, but its intracellular availability is often limited due to the competition with essential cellular metabolism. Several malonyl-CoA biosensors have been developed for high-throughput screening of targets increasing the malonyl-CoA pool. However, they are limited for use only in and and require multiple signal transduction steps.

Metabolic engineering of Escherichia coli for high-level astaxanthin production with high productivity.

Astaxanthin is a reddish keto-carotenoid classified as a xanthophyll found in various microbes and marine organisms. As a powerful antioxidant having up to 100 times more potency than other carotenoids such as β-carotene, lutein, and lycopene, astaxanthin is a versatile compound utilized in animal feed, food pigment, health promotion and cosmetic industry. Here, we report development of metabolically engineered Escherichia coli capable of producing astaxanthin to a high concentration with high productivity.

CuiD is a crucial gene for survival at high copper environment in Salmonella enterica serovar Typhimurium.

Copper ion is an essential micronutrient but it is also extremely cytotoxic when it exists in excess. Our studies have shown that Salmonella enterica serovar Typhimurium can survive potentially lethal copper exposures by the way of copper efflux system. A copper ion inducible gene was identified in virulent S.