Differential expression profiling of onco and tumor-suppressor genes from major-signaling pathways in Wilms' tumor.

Purpose: Wilms' tumor is the most-frequent malignant-kidney tumor in children under 3-4 years of age and is caused by genetic alterations of oncogenes (OG) and tumor-suppressor genes (TG). Wilms' tumor has been linked to many OG-&-TG. However, only WT1 has a proven role in the development of this embryonic-tumor.

Development, standardization and validation of molecular techniques for malaria vector species identification, trophic preferences, and detection of .

Background & Objectives: Knowledge on prevalence of malaria vector species of a certain area provides important information for implementation of appropriate control strategies. The present study describes a rapid method for screening of major Anopheline vector species and at the same time detection of Plasmodium falciparum sporozoite infection and blood meal preferences/trophic preferences.

Methods: The study was carried from February 2012 to March 2013 in three seasons, i.

A shift in resting habitat and feeding behavior of Anopheles fluviatilis sibling species in the Keonjhar district of Odisha, India.

Background: Anopheles fluviatilis exists as a complex of sibling species S, T, U and V exhibiting distinct variations. Sibling species S is considered as the main vector and anthropogenic whereas T, U and V are zoophagic non-vectors. This study was performed in a forested village of Keonjhar district, Odisha to identify the status of An.

Vectorial capacity and genetic diversity of Anopheles annularis (Diptera: Culicidae) mosquitoes in Odisha, India from 2009 to 2011.

Anopheles annularis is one of the major vectors of malaria in Odisha, India. The present study was undertaken to determine the vectorial capacity and assess the genetic diversity of An. annularis collected from different endemic regions of Odisha.

Genetic characterization of E2 region of Chikungunya virus circulating in Odisha, Eastern India from 2010 to 2011.

Chikungunya virus (CHIKV) infection has caught attention yet again as it rages around the globe affecting millions of people. The virus caused epidemic outbreaks affecting more than 15,000 people in Odisha, Eastern India since 2010. In this study, complete genetic characterization of E2 gene of CHIKV circulating in Odisha from 2010 to 2011 was performed by virus isolation, RT-PCR, molecular phylogenetics and bioinformatics methods.

Anopheles culicifacies sibling species in Odisha, eastern India: First appearance of Anopheles culicifacies E and its vectorial role in malaria transmission.

Objective: To identify the Anopheles culicifacies sibling species complex and study their vectorial role in malaria endemic regions of Odisha.

Methods: Mosquitoes were collected from 6 malaria endemic districts using standard entomological collection methods. An.

Molecular investigations of dengue virus during outbreaks in Orissa state, Eastern India from 2010 to 2011.

Dengue is one of the most important arboviral diseases in India. Orissa state in Eastern India reported the first dengue outbreak in 2010, followed by extensive outbreaks in 2011, affecting large number of people. Detailed entomological, serological and phylogenetic investigations were performed in mosquitoes and patients serum collected from dengue virus (DENV) affected areas of Orissa.

Molecular investigations of chikungunya virus during outbreaks in Orissa, Eastern India in 2010.

Chikungunya virus (CHIKV), an arthritogenic alphavirus, is transmitted to humans by mosquitoes of genus Aedes, mainly Aedes aegypti and Aedes albopictus. The resurgence of CHIKV in different parts of India is a point of major public health concern. In 2010, chikungunya outbreaks with high epidemic magnitude were recorded in coastal areas of Orissa, Eastern India, affecting more than 15,000 people coupled with severe arthralgia and prolonged morbidites.

Development and evaluation of a single-step multiplex PCR to differentiate the aquatic stages of morphologically similar Aedes (subgenus: Stegomyia) species.

Objective: To develop a single-step multiplex PCR to differentiate the aquatic stages of Aedes aegypti, Aedes albopictus and Aedes vittatus collected from different breeding spots in arbovirus endemic/epidemic areas and to detect the most abundant species by the multiplex PCR.

Methods: Aquatic stages of different mosquito species were sampled by inspecting artificial and natural breeding sites in domestic and peridomestic areas. DNA was isolated from different stages of the three Aedes species.